alexa TB Vaccines: The (Human) Challenge Ahead | OMICS International
ISSN: 2161-1068
Mycobacterial Diseases

Like us on:

Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

TB Vaccines: The (Human) Challenge Ahead

David A Hokey*

Department of Immunology, Aeras, Rockville, Maryland, USA

Corresponding Author:
David A Hokey
Director of Immunology, 1405 Research Boulevard, Suite 110
Rockville, MD 20852, Maryland, USA
Tel: 240-599-3077
Fax: 301-547-2987
E-mail: [email protected]

Received Date: February 02, 2014; Accepted Date: February 21, 2014; Published Date: February 25, 2014

Citation: Hokey DA (2014) TB Vaccines: The (Human) Challenge Ahead. J Mycobac Dis 4:e128. doi:10.4172/2161-1068.1000e128

Copyright: © 2014 Hokey DA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Mycobacterial Diseases

Tuberculosis (TB) remains a global health threat, with 8.6 million new cases and 1.3 million deaths (including 0.3 million in HIV-positive people) in 2012 [1]. An estimated one-third of the world’s population is infected with Mycobacterium tuberculosis (Mtb) [2]. TB is one of the leading causes of death by an infectious disease worldwide, second only to HIV (1.6 million deaths in 2012), and the increase in drug resistant strains of Mtb is alarming [1,3]. An effective vaccine to prevent infection and/or pulmonary TB disease is desperately needed. However, there are several hurdles that have made TB vaccine research and development painfully slow, including the slow growth rate of Mtb, the lack of a predictive animal model, and the lack of an immune correlate [3]. In addition, animal challenge models are very expensive due to the requirement of an ABSL-3 facility, and clinical efficacy studies are long and require large numbers of patients. Funding continues to be an issue in the TB vaccine field (Figure 1) [1,4-6], particularly given the difficulties and the expense of preclinical and clinical studies. New tools are needed to evaluate candidate vaccines that produce a more rapid and accurate assessment of the potential for vaccine efficacy and, therefore, help to increase the speed, and reduce the cost, of vaccine candidate selection.


Figure 1: NIH funding for HIV, TB, and malaria. Pie charts represent the approximate proportion of deaths (in millions; left pie) and the level of NIH funding (in millions; right pie) for HIV (blue), TB (red), and malaria (green). Data is based on the Estimates of Funding for Various RCDC, Global Tuberculosis Report 2013, World Malaria Report 2013, and the Global Report: UNAIDS Report on the Global AIDS Epidemic 2013.

Given the absence of an immune correlate, functional assays that can assess the ability of a vaccine to inhibit mycobacterial growth are needed to give some measure of assurance of the efficacy of a vaccine candidate. There has been some progress in this area, such as the mycobacterial growth inhibition assay (MGIA), which is currently being developed by a consortium of international scientists and organizations. The MGIA has the potential to be a surrogate for protection and may lead to the identification of immune correlates [7,8]. However, it remains to be seen whether this in vitro assay correlates with any type of protection in humans.

Malaria vaccine development has experienced similar challenges to the TB vaccine field given the lack of an immune correlate [9,10]. While smaller clinical trials can assess the safety and immunogenicity of a vaccine, large and expensive efficacy studies generally are needed to assess whether the observed immune responses are able to impact disease. A human challenge model of malaria infection has helped malaria vaccine developers to overcome these obstacles and, in so doing, has transformed the malaria vaccine field, providing a relatively inexpensive and rapid method for assessing vaccine efficacy prior to the initiation of very expensive phase IIb and phase III clinical efficacy trials [10]. The malaria human challenge model involves the vaccination of healthy volunteers who are then subjected to experimental malarial infection by mosquito bite. The volunteers are followed until parasites are detected in the blood, at which point they are treated with antimalarial medication [10,11]. The malaria human challenge model has been shown to be safe with over 1,450 volunteers challenged by this method [10-12]. Results from the RTS,S trial appear to confirm the predictive value of the human challenge model [10].

The development of a human challenge model for tuberculosis is not as straight forward as for malaria. While there are ethical concerns for any human challenge model [13], exposing healthy individuals to virulent Mtb poses significant safety concerns and is ethically unacceptable. One possible alternative is to use the licensed vaccine for TB, Bacille Calmette-Guérin (BCG), as a surrogate for Mtb. BCG is a replication-competent, live attenuated mycobacterium derived from Mycobacterium bovis that offers limited and variable protection against disseminated forms of childhood TB. BCG is administered intradermally as a live vaccine that is cleared in immunocompetentvaccinees, eliciting similar immune responses to those observed following Mtb infection [14]. Because of its long safety record in healthy infants and intradermal administration, BCG is attractive for use for human challenge studies in healthy adults.

Helen McShane’s group at Oxford University developed a preclinical in vivo murine challenge model using BCG. In this model, they challenged mice previously administered a TB vaccine with an intradermal injection of BCG in the ear and measured the change in BCG burden over time. The authors demonstrated that the intradermal BCG challenge in the ear mimicked an intranasal challenge, suggesting this model is relevant for assessment of protection [14]. This work provided the basis for the development of a human challenge model. In 2012, Minassian et al. published data describing a human challenge study with BCG. The researchers challenged both BCG-naïve and BCG-vaccinated volunteers with intradermal BCG injections and subsequently collected biopsies and performed PCR and cultures to measure the number of bacteria present at the injection site. In addition, they collected fluid from suction cup blisters to examine cellular infiltrates and measured immune responses in the peripheral blood. The authors observed differences in the bacterial counts obtained from different volunteers that had been previously vaccinated with BCG, which they hypothesized could be due to varying levels of exposure to environmental mycobacteria. There were some concerns regarding the study. First, the PCR and culture counts were not in agreement, which could have been due to either PCR counting of dead bacteria or possibly due to differences in the time when samples were plated. In addition, because volunteers were enrolled over the course of two years, different batches of BCG were needed for vaccination, with batches having a variability of approximate 1-log of bacteria [15]. The results of this study suggest that human challenge with BCG is safe and feasible, although in need of further optimization.

While the potential for a BCG challenge model is encouraging, there are a few issues that must be addressed. The challenge strain should be capable of evaluating all, or most, of the vaccine candidates that are under development. While BCG has much in common with Mtb, there are differences, and vaccines that target antigens unique to Mtb would not be suitable for the BCG challenge model. Potential ways to circumvent this problem would be to use either a recombinant BCG that expresses the unique Mtb antigens or to use attenuated Mtb, which is currently being investigated as a vaccine candidate [16]. Ideally, the challenge model would be less invasive and not require the use of biopsies to acquire samples from volunteers. Prior studies examined swabbing the infection area in order to culture any shed bacteria from the surface [17]. It may also be possibly to utilize luminescence or other novel non-invasive methods to quantify bacteria at the challenge site. Additionally, the challenge dose must be standardized. If the dose range varies by a log or more, the ability to distinguish small protective responses is greatly diminished. Such differences ultimately may not impact the readout of the assay, but more work needs to be performed to specifically address this issue.

A study has been submitted to to examine dose escalation for the human BCG challenge model, with challenge doses ranging from 2-16×106 CFU, in order to assess safety, shedding, and reproducibility of shedding. The researchers will utilize PCR, culture, and quantitative MGIT BACTEC culture to assess bacterial shedding [18]. This study has not started as of this writing but the results may have a significant impact on the future of this model and may help to address some of the concerns regarding the variability of the challenge stock.

The human challenge model could change the field of TB vaccine development as the malaria human challenge model did for malaria vaccines, not only by providing a less expensive and rapid method for assessing potential vaccine efficacy, but also by permitting more rapid progress toward the identification of an immune correlate of protection. While the challenges in developing this model are great, it is clear that the research community is embracing this challenge.


This study was supported by the National Institute of Health grant R01 AI090691-03 (PI: Evans, Thomas).


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 11949
  • [From(publication date):
    August-2014 - Jul 16, 2018]
  • Breakdown by view type
  • HTML page views : 8162
  • PDF downloads : 3787

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7