alexa The Effect of Umbilical Cord Blood Mesenchymal Stem Cells on the Neurological Function and the Expression of Caspase-3 in Cerebral Ischemiareperfusion Rats | Open Access Journals
ISSN: 2161-1009
Biochemistry & Analytical Biochemistry
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The Effect of Umbilical Cord Blood Mesenchymal Stem Cells on the Neurological Function and the Expression of Caspase-3 in Cerebral Ischemiareperfusion Rats

Ming Zhang1#, Xiaomin Hua1#, Yudan Zhao2, Bin Wang2* and Yujun Xia1*

1Department of Anatomy, Qingdao University Medical College, Qingdao 266071, People’s Republic of China

2Department of Microbiology, Qingdao University Medical College, Qingdao 266071, People’s Republic of China

#These authors Contributed Equally to the work

*Corresponding Author:
Yujun Xia
Department of Anatomy, Qingdao
University Medical College
Qingdao 266071, People’s Republic of China
Tel: +86-13863976458
E-mail: [email protected]

Bin Wang
Department of Microbiology
Qingdao University Medical College
Qingdao 266071, People’s Republic of China
Tel: +86-13685326203
E-mail: [email protected]

Received Date: March 23, 2015; Accepted Date: April 16, 2015; Published Date: April 20, 2015

Citation: Zhang M, Hua X, Zhao Y, Xia Y, Wang B (2015) The Effect of Umbilical Cord Blood Mesenchymal Stem Cells on the Neurological Function and the Expression of Caspase-3 in Cerebral Ischemia-reperfusion Rats. Biochem Anal Biochem 4:170. doi: 10.4172/2161-1009.1000170

Copyright: © 2015 Zhang M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Cerebral infarction, a type of ischemic stroke disease, is a threat to human health. Thrombolytic therapy and brain protection methods have been used to treat cerebral infarction clinically. Recently, cell therapy has become a hotspot. This study aimed to explore the effect of Umbilical Cord Blood Mesenchymal Stem Cells on the neurological function and the expression of caspase-3 in cerebral ischemia-reperfusion rats. Methods: The rat cerebral infarction models were established. The treatment group was injected into 2×106 Umbilical Cord Blood Mesenchymal Stem Cells; Sham-operated group and the control were injected into the same amount of cell culture media. Evaluation of neurological function was performed and the mRNA and protein expression level of caspase-3 was detected using RT-PCR and immunohistochemical method, respectively. Apoptosis analysis was assessed using a TUNEL kit. Results: Labeled Umbilical Cord Blood Mesenchymal Stem Cells survived well after being injected into rats, while the green fluorescence signal was not discovered in the control and sham-operated group; The neurological function of rats in the treatment group improved compared to the control (p<0.05). RT-PCR results indicated that the expression level of caspase-3 significantly decreased in rats of the treatment group (P<0.01), while there was no significant difference between the sham-operated group and the control (P>0.05). Immunohistochemistry results indicated that the number of caspase-3 positive cells decreased in the treatment group than the control (P<0.01), while there was no significant difference between the sham-operated group and the control (P>0.05). Apoptosis results showed that TUNEL-positive cells were significantly lower in the treatment group than the control group. This study indicated that Umbilical Cord Blood Mesenchymal Stem Cells contribute to the recovery of neurological function of cerebral ischemia-reperfusion rats and the expression of caspase-3 decreased.

Keywords

Ligand; Ion; Cu; Determination; Reagent

Introduction

Cerebral infarction, accounting for 75% of cerebrovascular disease, is a threat to human health, and the mortality and disability rates are very high [1]. Recently, cell therapy has been a hotspot. However, many ethical issues, including the obtaining and culturing of neural stem cells, limit its application clinically. Umbilical Cord Blood Mesenchymal Stem Cells (UCB-MSCs) belong to pluripotent stem cell and improved the limb function in cerebral ischemia-reperfusion rats [2,3]. Studies have shown that UCB-MSCs are capable of amplification in vitro compared to the bone marrow-derived mesenchymal stem cells (BM-MSCs) [4,5]. UCB-MSCs have been the seed cells to be injected to treat cerebral infarction. The purpose of this study is to explore the effect of UCB-MSCs on the neurological function and the expression of caspase-3 in cerebral infarction rats.

Materials and Methods

Rats

All procedures described in the study were reviewed and approved by the Ethical Committee of Qingdao University, Qingdao City, China. Wistar, a widely used experimental strain, male, 2-month-old, clean grade, weight (250 ± 20) g, were provided by Qingdao Food and Drug Administration.

Grouping

81 wistar rats were divided into three groups randomly: UCB-MSCs Group (the treatment group), sham-operated group and the control group. Each group was divided into three subgroups: 1d, 3d, 7d, containing 9 rats per subgroup. 24 hours after the successful modeling, UCB-MSCs (1×107/mL) provided by Tsinghua university, were injected into rats. Sham-operated group was not treated.

Establishment of rat cerebral ischemia-reperfusion model

Rat cerebral ischemia-reperfusion model was established according to Longa’s methods [6]. After the animal regains total consciousness, neurological deficits can be evaluated by a simple scale (five-point scale) as follows:

0: no observable deficits

1: failure to extend contralateral forepaw (mild focal neurologic deficit)

2: circling in a direction contralateral to infarct (moderate focal neurologic deficit)

3: falling in a direction contralateral to infarct (severe focal neurologic deficit)

4: depressed level of consciousness without spontaneous movement

Scoring 2 and 3 were collected for subsequent trials. The right carotid artery of sham-operated group was exposed and sutured later.

Immunohistochemistry

After rats were anesthetized, brain tissues were sliced and the sections were chosen for immunohistochemical staining. Samples were then incubated with the primary antibodies followed by incubation with a secondary antibody (Bioss, China) for 30 minutes. The reaction was visualized using diaminobenzidine (DAB). Nikon ECLIPSE E600 computer was used for image analysis. The percentage of caspase-3- positive cells from each sample was calculated.

RT-PCR analysis

Total RNA was isolated and reverse-transcribed using RNA isolation Kit (Cwbiotech, China) and Real Time PCR Kits (Takara, China) according to the manufacturer’s instructions, respectively. PCR was performed using the following PCR primers: F-TGGAACAAATGGACCTGTTGACC- 3, R-AGGACTCAAATTCTGTTGCCACC-3. Semi-quantitative analysis was performed using ID Image Analysis software.

Apoptosis analysis

Apoptotic cells in frozen brain tissues were detected using DeadEndTM Fluorometric TUNEL System (Promega, USA) according to the manufacturer’s instructions.

Statistical analysis

All statistical analysis was performed using SPSS 11.5. All data were performed using Student’s t-test and one-way ANOVA. P<0.05 was considered statistically significant.

Results

Labeled UCB-MSCs distributed in hippocampal CA1 region

Green fluorescence labeled UCB-MSCs were visible in hippocampal CA1 region under the microscope, suggesting that UCB-MSCs were injected into the rat brain tissues of the treatment group and survived well, while the green fluorescence signal was not discovered in the control and sham-operated group (Figure 1).

biochemistry-analytical-biochemistry-hippocampal

Figure 1: Green fluorescence labeled UCBMSCs distributed in rat hippocampal CA1 region of the treatment group (×100) (a: 1d; b: 3d; c: 7d).

Comparison of neurological function

Neurological dysfunction was not found in the sham-operated group, while there was apparent limb dysfunction in the control group, which revived mostly 2 hours after the operation. Compared to the control group, there was no significant improvement in the 1d treatment group (P>0.05). The score decreased in the 7d surgery group (1.333 ± 0.816) than the control group (2.167 ± 0.408), and the difference was statistically significant (P<0.05) (Figure 2A).

biochemistry-analytical-biochemistry-neurological

Figure 2: (a) Comparison of neurological function in three groups; (b) Grey level of caspase-3 mRNA in three groups; (c) Comparison of caspase-3 immunohistochemical staining positive cells; d Comparison of TUNEL-positive cells in the treatment group and the control.

The effect of UCB-MSCs on the mRNA expression level of caspase-3 in ischemia-reperfusion rats

The mRNA expression level of caspase-3 in subgroups of the treatment group decreased compared to the control group, and the difference was statistically significant (P<0.01) (Figure 2B).

Immunohistochemistry analysis

Immunohistochemistry results indicated that the rate of caspase-3 positive cells in control group was higher than that of the sham-operated group (P<0.01), while the rate of caspase-3 positive cells in subgroups of the treatment group decreased compared to the control group, and the difference was statistically significant (P<0. 01) (Figures 2C and 3).

biochemistry-analytical-biochemistry-Immunohistochemical

Figure 3: Immunohistochemical staining of caspase-3 and the caspase-3 positive cells were observed (×100) (a: the control group; b: the treatment group; c: the sham-operated group)

Apoptosis detection

Apoptosis results showed that TUNEL-positive cells were significantly lower in the treatment group than the control group (P<0.01) (Figure 2D).

Discussion

A cerebral infarction is a type of ischemic stroke resulting from a blockage in the blood vessels supplying blood to the brain [7]. We established rat cerebral ischemia-reperfusion models to imitate cerebral infarction in this study. Cerebral ischemia-reperfusion injury is a complex pathophysiological process. Linnik confirmed that neuronal apoptosis involved in cerebral ischemia-reperfusion injury [8]. Therefore, preventing neuronal apoptosis can reduce the brain damage effectively. Recent studies have shown that the activation of caspase-3 is a key step involved in neuron apoptosis after cerebral ischemia [9].

Currently, MSCs (mesenchyal stem cells) transplantation is prevalent in the field of cell therapy of cerebral infarction. Chen found that MSCs can significantly reduce neurological dysfunction in MCAO rats, but as he grew older, the amplification and differentiation of MSCs decreased significantly [10]. Recent studies have confirmed that MSCs also exist in UCB-MSCs, easy to access and no ethical issues. So we chose UCB-MSCs to treat cerebral ischemia-reperfusion injury in rats. This result indicated that the neurological function of UCB-MSCs treated rats improved significantly than the control group. However, the control group without UCB-MSCs injection improved slightly and it may be related to brain plasticity and natural restoration. And the number of neuronal apoptosis in the treatment group rat also decreased significantly compared to the control group. This shows that UCBMSCs play an important role in cerebral ischemia-reperfusion injury of the treatment rats.

Caspase-3, a homologous cysteine proteinase, also known as death protease, plays an important role in regulating cell apoptosis. Studies show that the expression level of caspase-3 is low in normal adult rat brain tissues and increased significantly after cerebral ischemia [11,12]. Le also showed that the rate of TUNEL-positive cells decreased by 36% in cerebral ischemia-reperfusion rats compared with normal and caspase-3 inhibitors can effectively reduce the number of apoptotic cells [13,14]. Therefore, growing evidence indicate that caspase-3 participates in cerebral ischemic injury and plays an important role in the neuronal apoptosis.

In conclusion, the present study found that UCB-MSCs improved the neurological function and reduced the transcription and translation level of caspase-3 in cerebral ischemia-reperfusion rats, especially the number of apoptotic cells. These results provide a novel insight into the influence of the UCB-MSCs to cerebral infarction. However, the effects of UCB-MSCs on the caspase-3 pathway and its therapeutic effect to cerebral infarction remain to be further explored.

Acknowledgements

This work was financially supported by National Nature Science Foundation (81471958).

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