Tuberculosis Infection in Cattle and Cattle Owners in North Eastern Parts of Ethiopia

Introduction: Ethiopia is one of the highest TB burden countries in the world. Transmission of M. tuberculosis between humans is well known, however, little is known about the transmission of M. tuberculosis complex species between human and animals, in particular cattle. The purpose of this study was, therefore, to investigate the status of bovine tuberculosis infection in cattle owned by tuberculosis suspected households and isolate M. tuberculosis complex species from sputum of chronic coughers in North Wollo Zone of the Amhara National Regional State, Ethiopia. Method: A cross-sectional study was conducted in selected Kebeles of North Wollo Zone. An intradermal tuberculin skin test was carried out on 381 cattle and sputum samples collected from 124 chronic coughers. Sputum samples were cultured and positive ones were identified by polymerase chain reaction using RD4 and RD9 as a marker. Result: Of the 124 sputum samples cultured, 4/ 124 (3.2%) were positive. Polymerase Chain Reaction using has confirmed that 2/4 (50%) were found to be M. tuberculosis while the rest was atypical Mycobacterial species. Of the 381 tested cattle 5/381 (1.31%) and 10/38 (2.63%) were found positive according to manufacturer recommendation (with a cutoff value > 4 mms) and a cutoff value > 2 mms, respectively. About 67% (6/9) of the individuals who owned positive cattle were having the habit of drinking raw milk. Among the ten positives, five of them resided in lowland (Kolla) areas (1300-1500 mabs). However, none of the owners of tuberculin positive cattle were found to be TB positive. Conclusion: This study has shown 3.2% of culture positivity and of these two were found to be M. tuberculosis. The sputum sample culturing from suspected chronically coughing individuals would help detection of the TB causative agents. According to the current findings BTB is considerable in Ethiopia and cattle should be seen as a potential source of zoonotic TB to humans. Bi olo gy a nd Micine ISSN: 0974-8369 Biology and Medicine Citation: Mengistu A, Enquselassie F, Mulatu F, Hailu E, Beyene D (2015) Tuberculosis Infection in Cattle and Cattle Owners in North Eastern Parts of Ethiopia. Biol Med (Aligarh) 7: 241. doi: 10.4172/0974-8369.1000241 Volume 7 • Issue 4 • 1000241 Biol Med (Aligarh) ISSN: 0974-8369 BLM, an open access journal Page 2 of 7 conducted from December 5-24/2012. Study population, sampling method and sample size In this study, individuals suffering from chronic cough for a duration of two or more weeks were suspected as tuberculosis cases [15,16]. Sputum samples were collected from TB suspected individuals who own cattle and comparative cervical intradermal tuberculin test (CCITT) were conducted on their cattle. Single population proportion sample size determination was used, with the assumptions, prevalence of M. tuberculosis in herds recorded in Sudan [17,18] of 7.4% with 3% precision and 95% confidence interval. Based on this, 314 bovines were required to be tested. Gidan, Gubalafto, Rayakobo and Meket Woredas were selected purposefully and 314 cattle’s were distributed proportional to sample size to each Woreda. Based on these 39, 79, 79 and 117 cattle were planned to be tested in Gidan (Eyela Kebele), Gubalafteo (Woynye and Sibilkay Kebeles), RayaKobo (Addis-alem and Robit area Kebeles) and Mekete (033 and 044 Kebeles) Woredas, respectively. Assuming an average of 3 cattle owned by a household in Amhara region, approximately 105 households were required to participate in the study. The seven Kebeles listed above (study sites), were selected based on the report having a high TB occurrence or being suspected to have TB by the local authorized personnel. Intradermal skin test on cattle Comparative cervical intradermal tuberculin test was conducted as manufacturer’s recommendation [19]. The operation was performed on either side of the animal middle neck region. Two circular areas of about 2.5cm diameter, about 12–15 cm apart, on the cervical area of the skin, were shaved using a scalpel blade. The initial skin thickness was measured with a digital caliper [20]. Measurements were read to the nearest millimeter. Lelystad (Netherlands) products of 0.1 ml (20 000 IU/ml) of bovine tuberculin PPD at one site and 0.1 ml (25 000 IU/ml) of avian tuberculin PPD at the other site were injected into the dermis using BD1/2 tuberculin syringe [21]. Correct injection was confirmed by palpating a small pea-like swelling at each site of injection. Skin thickness was measured again at both injection sites after 72 hrs + 4 hrs. The reaction at each site was derived by calculating the difference of the two tests. The results were interpreted according to the manufacturer recommendations [19] using the cutoff point for positivity of the test, that is >4 mms difference, calculated as the difference between skin thicknesses of bovine tuberculin (B) and avian tuberculin (A) injection sites (B – A). But, Ameni et al. [22] reported >2 mm difference as positive in Ethiopia. Based on his report, the test was considered positive if the difference was greater than 2 mm. Cattle body weight estimation For the purpose of this study the method mentioned by Nicholson and Butterworth [23] with modifications was used to determine the body condition of the zebu cattle. Sputum sample collection and processing For M. tuberculosis isolation sputum samples were collected from individuals suffering from chronic cough, according to Cheesbrough [24]. Suspected cases were asked to produce an “on spot” sputum specimen in a 20 ml screw plastic container under the supervision of a trained laboratory technician in the open air. Those cases who produced sputum were asked to give additional sputum samples on the second day and then on the spot. The sputum samples obtained from each patient was put in an ice box with ice packs (4°C), and immediately after collection, it was transferred to the hospital laboratory to be stored at -20°C. The samples collected from each site transported to Armauer Hansen Research Institute/AHRI TB laboratory to be processed. Briefly, an equal amount of Phosphate-buffered saline solution was added to the sputum and then decontaminated, digested with equal volume of 4% sodium hydroxide for 15 minutes. Then vortaxing was done in a closed tube till the mixture becomes homogeneous. The homogenate was centrifuged at 3000 RPM for 15 minutes. Neutralization done by using concentrated hydrochloric acid. In order to monitor neutralization 1 or 2 drops of phenol red was added. The supernatant was decanted and the sediment was inoculated onto Lowenstein-Jensen medium (tubes containing glycerol and sodium pyruvate) using the drop method. The tubes were incubated at 37°C and examined for growth weekly for 12 weeks [25,26]. Cultures were considered negative if there was no mycobacteria growth after 12weeks of incubation. Positive cultures were sub-cultured onto another set of media and incubated for another 3–4 weeks for further identification. Polymerase chain reaction using RD4 and RD9 as a marker was conducted. Armauer Hansen Research Institute (AHRI) M. tuberculosis (H37Rv) and M. bovis (AHRI No.1632) isolates used as a positive control. Data management, entry, analysis and data quality Good data management practices such as study monitoring, timely data archiving and transferring, sorting and filing were done. The collected data were coded and entered into EpiInfo version 3.2.1 [27]. Data cleaning and analysis were done by using SPSS software version 16.0 [28]. Descriptive statistics like frequency distributions, percentages, mean and standard deviations were used to describe the findings. Tables were used to present the results. The chi-square test was conducted to assess associations. All statistical tests were two-sided and significance was considered at a p-value <0.05. All data were collected in collaboration with experts and checked regularly while collecting the data by the principal investigator. All tuberculin tests were conducted by the same person and sputum sample collections and culturing done by laboratory personnel.


Introduction
Human TB, caused by M. tuberculosis, is among the oldest diseases in the world and is the seventh leading cause of death worldwide [1]. WHO [2] reported that 98% of tuberculosis deaths occurred in the developing world, affecting mostly young adults in their productive ages. Despite efforts made, globally or nationally to curb the effects of TB, different studies have shown that the trend of pulmonary TB remained as a health problem in the world [3][4][5][6][7]. In 2013, WHO [8] reported a decline in the impact of tuberculosis by the year 2012. The challenge of TB is becoming complicated by the occurrence of HIV/ AIDS, the emergence of multi-drug resistant TB and extremely multidrug resistant and to some extent with totally multidrug resistant strains reported in some parts of the globe [9]. TB is still killing nearly 5000 people every day and 1.8 million deaths in 2008 alone [10]. If TB is left unchecked within 20 years, it will kill 35 million people [9].
Ethiopia ranks third in Africa, 8 th among the 22 highest TB burdened countries in the world and the prevalence of all forms of TB is estimated at 261 per 100 000 population, leading to an annual mortality rate of 64 per 100 000 population [11]. In 2008 the Amhara National Regional State Health Bureau reported 1.6% and 3.5% TB case detection and TB death rates, respectively [12]. The sources of infection for M.tuberculosis are mainly those humans who are developing active TB [13]. However, M.tuberculosis has been isolated from different animal species, including bovines/cattle, which should be seen as a challenge to further move in the prevention or controlling effort made to tackle the disease. In Ethiopia, little is known about the transmission of M.tuberculosis complex from cattle to humans. The purpose of this study is therefore, to investigate/determine the prevalence of BTB and see the possible role of cattle in the epidemiology of human TB and isolate Mycobacterium tuberculosis complex (MTC) species in North Wollo Zone, the Amhara National Regional State.

Study population, sampling method and sample size
In this study, individuals suffering from chronic cough for a duration of two or more weeks were suspected as tuberculosis cases [15,16]. Sputum samples were collected from TB suspected individuals who own cattle and comparative cervical intradermal tuberculin test (CCITT) were conducted on their cattle. Single population proportion sample size determination was used, with the assumptions, prevalence of M. tuberculosis in herds recorded in Sudan [17,18] of 7.4% with 3% precision and 95% confidence interval. Based on this, 314 bovines were required to be tested. Gidan, Gubalafto, Rayakobo and Meket Woredas were selected purposefully and 314 cattle's were distributed proportional to sample size to each Woreda. Based on these 39, 79, 79 and 117 cattle were planned to be tested in Gidan (Eyela Kebele), Gubalafteo (Woynye and Sibilkay Kebeles), RayaKobo (Addis-alem and Robit area Kebeles) and Mekete (033 and 044 Kebeles) Woredas, respectively. Assuming an average of 3 cattle owned by a household in Amhara region, approximately 105 households were required to participate in the study. The seven Kebeles listed above (study sites), were selected based on the report having a high TB occurrence or being suspected to have TB by the local authorized personnel.

Intradermal skin test on cattle
Comparative cervical intradermal tuberculin test was conducted as manufacturer's recommendation [19]. The operation was performed on either side of the animal middle neck region. Two circular areas of about 2.5cm diameter, about 12-15 cm apart, on the cervical area of the skin, were shaved using a scalpel blade. The initial skin thickness was measured with a digital caliper [20]. Measurements were read to the nearest millimeter. Lelystad (Netherlands) products of 0.1 ml (20 000 IU/ml) of bovine tuberculin PPD at one site and 0.1 ml (25 000 IU/ml) of avian tuberculin PPD at the other site were injected into the dermis using BD1/2 tuberculin syringe [21]. Correct injection was confirmed by palpating a small pea-like swelling at each site of injection. Skin thickness was measured again at both injection sites after 72 hrs + 4 hrs. The reaction at each site was derived by calculating the difference of the two tests. The results were interpreted according to the manufacturer recommendations [19] using the cutoff point for positivity of the test, that is >4 mms difference, calculated as the difference between skin thicknesses of bovine tuberculin (B) and avian tuberculin (A) injection sites (B -A). But, Ameni et al. [22] reported >2 mm difference as positive in Ethiopia. Based on his report, the test was considered positive if the difference was greater than 2 mm.

Cattle body weight estimation
For the purpose of this study the method mentioned by Nicholson and Butterworth [23] with modifications was used to determine the body condition of the zebu cattle.

Sputum sample collection and processing
For M. tuberculosis isolation sputum samples were collected from individuals suffering from chronic cough, according to Cheesbrough [24]. Suspected cases were asked to produce an "on spot" sputum specimen in a 20 ml screw plastic container under the supervision of a trained laboratory technician in the open air. Those cases who produced sputum were asked to give additional sputum samples on the second day and then on the spot. The sputum samples obtained from each patient was put in an ice box with ice packs (4°C), and immediately after collection, it was transferred to the hospital laboratory to be stored at -20°C. The samples collected from each site transported to Armauer Hansen Research Institute/AHRI TB laboratory to be processed.
Briefly, an equal amount of Phosphate-buffered saline solution was added to the sputum and then decontaminated, digested with equal volume of 4% sodium hydroxide for 15 minutes. Then vortaxing was done in a closed tube till the mixture becomes homogeneous. The homogenate was centrifuged at 3000 RPM for 15 minutes. Neutralization done by using concentrated hydrochloric acid. In order to monitor neutralization 1 or 2 drops of phenol red was added. The supernatant was decanted and the sediment was inoculated onto Lowenstein-Jensen medium (tubes containing glycerol and sodium pyruvate) using the drop method. The tubes were incubated at 37°C and examined for growth weekly for 12 weeks [25,26]. Cultures were considered negative if there was no mycobacteria growth after 12weeks of incubation. Positive cultures were sub-cultured onto another set of media and incubated for another 3-4 weeks for further identification. Polymerase chain reaction using RD4 and RD9 as a marker was conducted. Armauer Hansen Research Institute (AHRI) M. tuberculosis (H37Rv) and M. bovis (AHRI No.1632) isolates used as a positive control.

Data management, entry, analysis and data quality
Good data management practices such as study monitoring, timely data archiving and transferring, sorting and filing were done. The collected data were coded and entered into EpiInfo version 3.2.1 [27]. Data cleaning and analysis were done by using SPSS software version 16.0 [28]. Descriptive statistics like frequency distributions, percentages, mean and standard deviations were used to describe the findings. Tables were used to present the results. The chi-square test was conducted to assess associations. All statistical tests were two-sided and significance was considered at a p-value <0.05. All data were collected in collaboration with experts and checked regularly while collecting the data by the principal investigator. All tuberculin tests were conducted by the same person and sputum sample collections and culturing done by laboratory personnel.

Exclusion criteria
Individuals having unproductive cough, less than 2weeks duration of cough, those who have no cattle as well as under TB treatment and below 15 years were excluded. If sputum was not produced within 15 minutes he/she was exempted. Female cattle either recently delivered or at their last trimester, newly added, and those less than 6 months of old as well as aggressive cattle were not included in the study.

Ethical considerations
Ethical clearance was obtained from the college of health sciences, Addis Ababa University institutional review board (IRB) and AHRI/ ALERT (Armauer Hansen Research Institute) ethics committee. Permission was obtained from the Amhara Regional Health Bureau. The aim of the study was explained to the patient who was complaining chronic cough with one or more additional clinical signs and his/her willingness were asked to participate in the study. If he/she is willing to participate, consent were taken and sputum samples were collected. Confidentialities were maintained and to maintain confidentiality codes was used rather than participants' names. Based on the results individuals who own positive cattle were advised and those individuals whose sputum samples were cultured positives communicated through health institutes to receive appropriate treatments and conduct family screening.
The mean age of participants was 50 (± 15) and nearly one third of them aged between 40-50 years. The majority (nearly 60%) of the individuals was having family size ranging from 1-5years age. About 92% of the participants reported to be followers of Orthodox Christianity. Of the participants about 69.4% were illiterate Table 1.
As it is presented in Table 2, among the participants, 17.3% and 15.3% had a TB history in their household and the patients were received antituberculosis treatment, respectively. Among the treated ones, only 15.3% completed a course of antituberculosis treatment. Chronic cough is not seen as a serious problem in the study area and hence nearly 77% of them were not taking any action and only 1% was seeking modern treatment for their ailments. Nearly 60% of the participants were suffering from chronic cough for more than 2 months. Nearly three fourth (73.4%) and more than half (nearly 52%) of the participants had the habit of taking unboiled milk and raw meat, respectively. Of the participants only 12% were having contact with TB diagnosed patients. Although there is no association all culture positive individuals were having the habit of taking unboiled milk as well as raw meat. From the collected 124 TB suspected sputum samples 4 Mycobacterial species, which accounted about 3.2%, were grown in LJ media. Polymerase chain reaction using deletion method revealed that 2/4 (50%) of the isolates were M. tuberculosis. Distribution of sputum culture positive results with some predictors is presented in Table 3. Table 3, all positive cultures (100%) were belonging to males and out of which 2 (50%) of them lived in the mid-altitude. Equal positive cultures were recorded in those who were illiterate and attending formal and informal education. Using the mean score (50yrs as a mean), three fourth of the culture positives where aged beyond 50 years. Besides, three fourth of the culture positives had a cough duration of more than 1month and 75% of them took traditional treatments to curve cough situation. All culture positive individuals were not having a TB history in their house and there was no contact history. Polymerase Chain Reaction (PCR) using RD 9 as a marker indicated that 2 (50%) among 4 sputum culture positives were M. tuberculosis while the rest were atypical Mycobacterial species.

As indicated in
Of the total participants who allowed their cattle to be tested 73.4% and 51.6% of them consumed unboiled milk and uncooked meat, respectively. In the actual work 381 cattle owned by these households were tested intra-dermal by using APPD (Avian purified protein derivative) and BPPD (Bovine purified protein derivative). Of the total cattle tested males contribute 192 (50.4%). The mean age of cattle was 6.0341 ± 3.5246 and the majority of cattle 63.5% (almost two-third)  *: refers to indigenous zebu cattle and crosses of Holstein-Frisian in the study area **: refers to the origin of cattle to the owner, ***: indicates the nutritional status of cattle during the time of testing ****: indicates the three major agro-climatic Zones in North Wollo Zone 1 differences in skin fold measurement greater than 2mms; 2 differences in skin fold measurement ranges between greater 1 mms-<2 mms; 3 differences in skin fold measurement less than 1 mms 4 Dega: equivalent to high land area; 5 WoinaDega: Equivalent to Mid altitude; 6 Kolla: equivalent to low land were having medium body condition. Almost 96% of the cattle tested were local zebu and 75% of them were home-bred (Table 4).
Based on manufacturer recommendation [19] 5 (1.31%) cattle were found positive. As it is shown in Table 4, according to previous cut off proved by Ameni et al. [22] of the tested cattle 10 (10/381) (2.63%) were positive for the test. Highest positivity (with a reading >4 mms) was recorded in Raya-Kobo Woreda, in Adis alem Kebele, where 5 (~8. 8%) cattle revealed BTB out of 57 tested cattle. The total households in the Adisalem Kebele were 312, of which 14 households (4.5%) were reported as having chronic cough and of these reactor cattle were recorded in four households, which is about 28.8%. According to the manufacturer recommendation 12 (about 3.2%) inconclusive test results were obtained. Of the positives females contribute 50% of skin test positivity. In general, according to Ameni et al. [22] report among the ten positive cattle, 4 (40%), 1 (10%) and 5 (50%) cattle were positive for the test in Dega, Woina Dega and Kolla Woredas, respectively.
From the tested cattle nearly two-third (63.5%), and among the positive once 60% of them were having medium body condition. Lean cattle did not show positive reaction. The majority of cattle (359/381), which accounted about 94.2%, were negative for the test. Excluding inconclusive test result cattle, Fisher's exact test (Table 4) showed a significance difference (P<0.05, χ 2 = 12.13 with 2 df) in skin test reaction among different agro-climatic Zones.

Bovine tuberculosis
According to the Purified Protein Derivative manufacturer's recommendation [19] from 381 tested cattle 5 of them showed positive results, in particular in Rayakobo Woreda, Addis-alem Kebele, which is characterized by kolla (equivalent to low land) agroclimatic Zone and this, contributes about 1.31%. Based on this, the prevalence of BTB in this study was 0.013 (95% CI, 1-03%). In another scenario when the cut of skin fold measurements is taken as >2 mms, according to Ameni et al., [22] the number of positive cattle recorded lifted to 10, which in turn raises the prevalence to 2.63%. Six (6/9, nearly 67%) individuals among nine who owned positive cattle and reported having a chronic cough were having the habit of drinking unboiled milk.
Of 124 sputum samples collected from chronic coughers 4 (3.2%) culture positives (2 of them were M. tuberculosis based on PCR), were isolated. A tuberculosis survey conducted from October 2010-June 2011 in Ethiopia, indicated 0.2% (110/51667) culture positivity from pulmonary TB suspected cases [48]. A study conducted in the rural community of South-west Ethiopia revealed a culture positivity of ~4% (17/482) [49]. The current result lies within the limits of the two findings. The variability between these results might arise from differences in the quality of sputum sample, time of sputum sample collections, cold chain systems during collections and transportations as well as the time of processing after collection. Fifty percent of the isolates confirmed as atypical Mycobacterial species. As it is indicated in literature people could be infected with atypical Mycobacteria or Mycobacteria other than tuberculosis (MOTT) and some of them could lead to disease development in different parts of the body including the lungs [50].
Majorities suffered from a chronic cough for more than 2 months and more than three-fourth (77%) of participants did not take any action to cure their cough as well as 75% of the culture positives had cough duration of more than 2 months. These factors will increase the chance of disease transmission; if the individual is infected with tuberculosis since smear negative-culture positive cases are responsible for 20% of tuberculosis transmission [51,52]. Three-fourth of the culture positive individuals were aged beyond 50 years old and this might be related to the decline in host immunity as age increases. Chain and colleagues 1995 [53] reported that immunologic abnormalities in elders led to tuberculosis development.
Among the coughing individuals, 74 % (nearly three fourth) were having the habit of taking raw/unboiled milk. Taking unboiled/raw milk could be a potential source of infection to humans as described above since infected cows excrete M. bovis via milk [54]. Polymerase chain reaction conducted from Albanian milk samples in Tirana university revealed the presence of the MTC gene in 36/50 (72%) of the tested samples would support the possibility of ingesting the disease causing agent [55].
In the current study, 381 cattle owned by chronic coughers were tested and prevalence of 2.63 was recorded. The prevalence of BTB in Meskanmareko, Woldeya, Bakogazer Woredas and Bale mountains was 7.9%, 1.2%, 4.3% and 2.0%, respectively with an overall prevalence of 3.1% [56]. The prevalence in small scale dairy production systems ranges from 3.5% in Asella to 14.2% in Wolaita Sodo area [57,58]. The prevalence in intensive dairy farms ranged from 4.5%-73.6% in Desie area [59,60], whereas in the traditional management system, it ranged from 3.4% (West Wolega) to 22% (Kombolcha) [59,61]. The present finding is a bit higher to the findings of Woldeya area, otherwise, it is in agreement with the overall findings reported by Tschopp et al. [56] and fall within the ranges of her findings. The difference in Woldeya area might be attributed to the time difference in conducting intradermal skin test, the area covered as well as the sampling techniques. The current finding difference from different management systems might be described to the differences in breed types/composition, the way of management of cattle, herd size, the environment and the body condition of the cattle during testing time, which is related to feed availability.
All cattle showed a positive result for the test was local zebu breed and 100% home-bred, having a longer contact period (with a mean age of 6.0341 + 3.5246) to the household. Longer contact period might act as a source of infection to the family members as well as cattle including other animal species and at large to the community. As a result of longer duration of stay with the household, females, particularly lactating ones will serve as a permanent source of infection since it has been reported that a single cow can excrete sufficient viable mycobacteria in milk to make even pooled milk infective [54,64] and infection of the udder has been found in varying proportions of tuberculous animals as high as 5.4% [54,62].
The difference between Dega, WoinaDega and Kolla areas in BTB was significant (P<0.05, χ 2 = 12.13 with 2 df). Report in Ethiopia indicated that the prevalence is relatively high in the highlands, having an altitude of >2000masl [63]. Indeed the presence of difference in skin test positivity in geographical locations is described by another author [64]. However, this needs further study since altitude in human TB showed a higher TB prevalence in low altitudes [65,66]. Purposive sampling of the study areas, lack of a comparative study, focusing on chronic coughers as well as failure to resample negative chronic coughers could be seen as a limitation of this study.

Conclusion and Recommendations
Despite the current finding in this study looked small and lacked representativeness, the work can tell us the advantage of culturing since all of the culture positive chronic coughers were smear negatives. These culture positive ones are active pulmonary TB cases and hence the spread of the agent to the family or those who had/have contact with them is more likely. In this study four were found positive culture, of which half were atypical Mycobacteria. The finding of atypical Mycobacterial species in humans should be seen as a problem in the study areas. The presence of skin test positive cattle in different herds owned by different households should be seen as an indication as to the possible source of infection to humans, in particular zoonotic TB. In this study, more than two-third of the individuals suffered from chronic cough and owned positive cattle practiced consuming raw milk, whereby infected cows excrete the bacilli via milk chance of taking the BTB causative agent. A prevalence of 2.63% was recorded and those positive cattle might remain as a source of infection for the family, to the neighbors and at large to the community as well as to other animal species. Even though bovine TB was not found from culture positive chronic coughers, the current approach to see the BTB situation on their cattle owned by them will serve as a springboard for future possible cross infection assessment of Mycobacterial species to both populations.
Early detection of chronic coughers, identification of the causes of chronic cough, family members screening for TB, treatment for active pulmonary TB cases, repeated sputum sample collection and processing for the chronic coughers as well as a means of exclusion or culling of positive cattle to break the possible cycles of BTB in the study area and continual community awareness with further research based on altitudes are recommended. Besides, to have a better picture as to the prevalence of the disease among individuals who suffered from chronic cough case control study is suggested.