Water and Fish Quality of Aquaculture Pond Adjacent to Intensive Pesticides Application Agro-System
Received Date: Jun 01, 2018 / Accepted Date: Jul 23, 2018 / Published Date: Jul 31, 2018
Water and fish quality was specified for a private aquaculture pond localized at the north of Egypt during June- November 2017. The main traditional physicochemical parameters of water were evaluated. Moreover, the occurred levels of trace elements, polycyclic aromatic hydrocarbons (PAHs) and pesticide residues in water and fish of Nile Tilapia (Oreochromis niloticus) and grey Mullet (Mugil cephalus) were specified. The levels of total hardness, ammonia as N, sulfide, nitrite and phosphate were violated the water quality guidelines for the fish cultures. Trace metals of arsenic, cadmium, chromium, copper, nickel, lead and zinc were detected in all the water and fish tissue samples at remarkably high levels (but still below the maximum permissible levels established by WHO. From 15 PAHs analyzed, naphthalene, acenaphthene, phenanthrene, fluorene, anthracene, fluoranthene, benzo(a)anthracene and benzo(b)fluoranthene were detected in water at range of 0.05 μg/l and 0.71 μg/l, while phenanthrene, fluorene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, benzo(b)fluoranthene and dibenzo(a,h)anthracene detected in fish muscles at range of 0.18 μg/g-0.72 μg/g fresh wt. Residues of α- and ϒ- HCHs, endosulfan Ã‰Âª, endosulfan Ã‰ÂªÃ‰Âª, endosulfan sulfate, heptachlor, heptachlor epoxide, p,p´-DDE, chlorpyrifos, cypermethrin, cyfluthrin and fenvalerate pesticides were detected within the permissible levels in water at the average of 0.12 ng/l-2.04 ng/l. The same compounds were also detected in the fish (except endosulfan sulfate, cyfluthrin and fenvalerate) at ranges of 0.87 ng/l-10.21 ng/g fresh wt. of Tilapia and 0.95 ng/l-8.32 ng/g fresh wt. of Mullet muscles. The potential occurrence of chlorpyrifos, cypermethrin, cyfluthrin and fenvalerate residues might be attributed mainly to its intensive current application on the vegetables and fruits around the fish pond, rather than the persistent organochlorines which still occurred as environmental contaminants from many years ago. The quantified levels of PAHs and pesticide residues in Tilapia and Mullet fish tissues analyzed were below its maximum permissible levels.
Keywords: Aquaculture; Fish; Metals; Pesticides; Polycyclic aromatic hydrocarbons; Water
The aquaculture activity is exhibiting the strongest growth of any fisheries related activity in Egypt. It is considered as the only viable option for reducing the gap between production and consumption of fish. Most aquaculture activities are located in the Nile delta region. This sector is currently the largest single source of fish supply accounting for almost 65 percent of the total fish production of Egypt with over 99% produced from privately owned farms . Egypt’s aquaculture industry ranks number 10 worldwide and number two in Tilapia production, behind only China, with the total production of 705,490 tons in 2009, increased to 1.560 million tons in 2015 [2,3]. Currently, it's a boom has been occurring where two major aquaculture projects were recently established, one is the Berket Ghalioun fish farm includes 4,000 acres of fish ponds, nurseries and hatcheries, north of Kafr-Elshiek governorate and the other is along the East Suez Canal zone. At full production, it is expected to provide 70% of the country’s domestic fish consumption requirement .
Freshwater fish are the main aquaculture products in Egypt beside brackish water fish. The native species are Nile Tilapia (O. niloticus ) and grey Mullet (M. cephalus ). In Egypt, a number of studies have been carried out for monitoring trace metals, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and pesticide residues and their bioaccumulation in aquatic biota. Certain of the quantified pollutants namely; lead, cadmium and Benzo(a)pyrene were exceeded the permissible limits recommended by FAO for fish, whereas the pesticides residue levels reported in biota below FDA regulatory action levels [5-10].
Physicochemical characteristics of the fish cultures water play a vital role in primary productivity and growth of the ponds fish. Therefore, the water quality assessment is very important for implementation of the monitoring and remediation programs to minimize the risk promoted by hazardous substances in aquatic ecosystems . Agriculture wastes mainly pesticides, synthetic fertilizers and human wastes released directly by underground and/or surface drained water constitute the major sources of pollutants of the aquaculture fish ponds . The main water source for many fish aquaculture ponds located at the northern regions of Nile delta is the agricultural wastewater of intensive vegetable and fruit agricultural areas. Taking these considerations into account, the present investigation aimed to evaluate water quality of a represented private fish pond at the north of El-Behera governorate, Egypt by assessing its main traditional physicochemical parameters level including pH, dissolved oxygen, total dissolved solids, turbidity, total hardness, total organic matter, extractable organic matter, total of petroleum hydrocarbons, sulfide, sulfate, ammonia as N, nitrite, nitrate and phosphate. In addition to determining the potential occurred levels of trace metals, PAHs and pesticide residues in water and fish of the pond.
Materials and Methods
Study area and sampling procedures
The study was carried out at a private fish aquaculture pond laid at the west of Rosetta, El-Behera governorate. The pond area is 4146 m2 supplied with agricultural wastewater from the adjacent vegetable and fruit sandy fields. Duplicate water samples (2.5 L each) were collected monthly using empty organic solvent glass bottles. About one kg (8-18/kg)/sample of Nile Tilapia (O. niloticus ) and grey Mullet (M. cephalus ) fish were collected every two months from the pond during June to November 2017.
The reference standards of the tested metals; arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), nickel (Ni), lead (Pb) and zinc (Zn)). Polycyclic aromatic hydrocarbons (PAHs): Naphthalene, acenaphthylene, acenaphthene, phenanthrene, fluorene, anthracene, pyrene, chrysene (internal standard (i.s)), benzo(a)anthracene, fluoranthene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, benzo(g,h,i)perylene, indeno(1,2,3-cd)pyrene and dibenzo(a,h)anthracene. Pesticides standard of organochlorines (α, β and γ-HCHs, δ-HCH (i.s), p,p’-DDT, p,p’-DDD, p,p’-DDE, endosulfan isomers (ɪ,ɪɪ), endosulfan sulfate, aldrin, dieldrin, endrin, heptachlor and heptachlor epoxide), organophosphorus (chlorpyrifos) and pyrethroids (cypermethrin, cyfluthrin and fenvalerate) were purchased from Supelco (USA).
Samples preparation and analysis
Physicochemical parameters analysis of water: Water quality of the fish aquaculture pond under investigation was characterized by determining levels of its common physicochemical parameters. Measuring the levels of pH, dissolved oxygen (DO), total dissolved solids (TDS), turbidity, total hardness, total organic matter (TOC), extractable organic matter (EOM), total of petroleum hydrocarbons (TPHs), sulfide, sulfate, ammonia as N, nitrite, nitrate and phosphate were carried out according to the methods of APHA and US-EPA [13-15].
Determination of trace metals in water and fish: The represented water and muscle tissues of O. niloticus and M. cephalus fish samples were acid digested according to standard methods of US-EPA [16,17]. The digested filtrate samples were subjected to metals analysis using Inductively Coupled Plasma Optical Emission Spectroscopy (Agilent ICP-OES 5100 VDV).
Determination of polycyclic aromatic hydrocarbons and pesticides in water: Water sample (2L) was extracted with methylene chloride according to US-EPA methods [18,19] for PAHs and pesticides, respectively. The extract was dried and concentrated to a volume of 1 ml, then divided into two 0.5 ml. The first 0.5 ml final extract was subjected to PAHs analysis using gas chromatograph equipped with flame ionization detector (GC-FID) and mass spectrometer (thermo-scientific MS-ISQ, 2009). Injection port was 250°C with splitless injection for 3 min, then split mode at a split ratio of 1:10. TG-1MS columns (100% dimethyl polysiloxane 30 m, 0.32 mm i.d., 0.25 film thickness) and helium as a carrier gas (1 ml/ min) were used. Oven temperature program: 80°C initial temperature to 240°C with increasing rate of 7°C min-1 and then to 300°C at the rate of 3°C min-1, and held for 5 min at 300°C was applied for FID and MS detector lines. FID temperature; 300°C, mass transfer line temperature; 300°C and ion source temperature at 220°C. The mass spectrometer-electron ionization mode with electron energy of 70 eV and quadrupole mass analyzer at mass range of 50 amu-500 amu was applied. NIST library 2.0 was used for compound identification depending on the fragmentation pattern matching with the given mass spectrum.
For qualitative and quantitative analysis of the tested pesticides, 1 μl-2 μl of the second 0.5 ml final extract was injected using thermo scientific triplus auto sampler at splitless mode (3 min) then, split at ratio of 1:10. The flow of nitrogen as the carrier gas was 1 ml/min. injector temperature was 250°C. TG 5 MS column (95% dimethyl polysiloxane, 5% diphenyl polysiloxane, 30 m, 0.32 mm i.d., 0.25 μm film thickness) was used under the following temperature program: GC oven was started at initial temperature 60°C, increased with rate of 4°C min-1 to the final temperature at 300°C and hold for 3 min. Micro-electron capture detector (63 Ni-μECD) temperatures were 300°C and 310°C for ECD base and cell, respectively, with 35 ml N2 as make up gas.
Determination of polycyclic aromatic hydrocarbons in fish: The blended free powder homogenate using anhydrous sodium sulfate (ACS reagent grade) of the Tilapia and Mullet muscles (20 g fresh wt. each) was extracted and cleaned up based on the method of Yunker et al.  with some modifications. N-hexane used (to extract PAHs after lipid saponification with 50% KOH methanol solution) and 1:1 nhexane: dichloromethane mixture (for cleanup elution process through silica gel and alumina column chromatography). Qualitative and quantitative analysis of the PAHs were carried out using GC-FID and GC-MS as mentioned above for water analysis .
Determination of pesticides in fish: The dry homogenate of fish muscle was subjected for ultrasonic extraction method using sonicator water bath (Selecta, Spain), with pesticide residue grade solvents; acetone: n-hexane (1:1) mixture for extraction of the three pesticide groups (OCs, OPs and pyrethroids) [22-24]. Final concentrate of water or fish extract was subjected for estimation of the extractable organic matter (EOM) gravimetrically followed by clean-up procedure through florisil (60-100 U.S. mesh) column. Chromatographic analysis of the tested pesticides was carried out using GC/63Ni-μECD as mentioned above for water analysis .
Quality assurance procedure: The quality control procedures for the determination of trace metals, PAHs and OCPs in water and fish tissue samples were carried out by analysis of matrix spikes, duplicates, laboratory blanks. Instrumental external calibration of ICP-OES, GCFID, GC-MS and GC-μECD were performed prior to and in between the analysis of each batch of samples by using working standard solutions of the tested metals and organic components mixture within the appropriate concentration range for its detection. Internal calibration for the chromatographic analysis was also applied using internal standards of 40 pg/μl δ-HCH, and 20 ng/μl chrysene. Determination of the recovery percentages was performed for all target metals and organic compounds in order to assess the efficiency of the analytical operating procedures applied. These percentages were found at ranges of 85%-97%, 72%-88% and 64%-93% for the tested metals, PAHs and pesticides, respectively. Moreover, method detection limits (MDLs) of the tested parameters were determined and calculated at ranges: for metals; 0.01 mg/l-0.03 mg/l water and 0.04 mg/kg-0.07 mg/kg fresh wt. fish. PAHs; 1.5 μg/l-4.5 μg/l water and 0.28 μg/g-0.58 μg/g fresh wt. fish. Pesticides; 1.0 ng/l-3.4 ng/l water and 3.7 ng/g-6.5 ng/g fresh wt. fish.
Results and Discussion
Physico-chemical characteristics of water
Water quality of the investigated aquaculture pond was specified monthly from June to November 2017 by measuring its common physicochemical parameters including pH, turbidity, dissolved oxygen (DO), total dissolved solids (TDS), total hardness, total organic matter (TOM), extractable organic matter (EOM), total of petroleum hydrocarbons (TPHs), sulfide, sulfate, ammonia as N, nitrite, nitrate and phosphate. The obtained results listed in Table 1 found that the levels of most physical and chemical parameters determined were not violated the water quality guidelines for the fish pond culture. Whereas some of the parameters specified namely; total hardness, ammonia as N, nitrate and phosphate were beyond permissible limits of the water quality guidelines [26-28].
|Parameters||June||July||August||September||October||November||Desirable limits [26-28]|
|Ammonia as N||2.85||2.6||3.05||3.38||3.15||2.87||<0.05|
Table 1: Physico-chemical characteristics of water of the fish aquaculture pond localized between vegetable and fruit fields, June-November, 2017.
The results obtained are potentially attributed to the intensive application of organic manure, chemical fertilizers (e.g., urea (46% N), ammonium nitrate (33.5% N) and phosphate (12.5% P2O5)), especially during growing seasons (spring and summer) of the vegetables and fruits. The discharge of these fertilizer residues into the agricultural wastewater (as the main water feeding source for aquaculture ponds) can be leading to fluctuating the parameters measured in water at different levels through the sampling period. Moreover, the study area is located near to Rosetta Mediterranean coast. This allows for seeping of the underground seawater toward the agricultural fields, mainly during the shortage of irrigation intervals in this area after fruits harvesting period. This might reflect the increase of water TDS from 585 mg/l in early summer to 720 mg/l during the autumn season.
Certain essential and non-essential elements were detected in the all water and fish samples analyzed as shown in Figures 1-3. The detected levels in water of As, Cd, Cr, Cu, Ni, Pb and Zn were at ranges of (0.19-0.28) μg/l, (0.27-0.40) μg/l, (1.18-1.50) μg/l, (128-165) μg/l, (1.34-1.92) μg/l, (1.14-1.70) μg/l and 351-406 μg/l. These metals were at levels of (0.36-0.52) μg/g, (0.70-0.85) μg/g, (1.33-2.18) μg/g, (2.81-4.02) μg/g, (0.36-0.43) μg/g, (0.65-0.77) μg/g and (7.72-10.45) μg/g fresh wt. of Tilapia fish tissues, whereas in Mullet at ranges of (0.24-0.28) μg/g, (0.57-0.76) μg/g, (1.21-2.34) μg/g, (3.34-3.66) μg/g, (0.31-0.38) μg/g, (0.55-0.90) μg/g and (6.15-8.95) μg/g fresh wt., respectively.
The study reveals that the Zn was found at higher levels in Tilapia than in Mullet fish muscles analyzed. The variations for metal levels through the different months of sampling period might be attributed to a fluctuation of the agricultural wastewater quality concerning levels of certain soil and foliar chemical fertilizers, fungicides containing metals as well as mineralized organic matter discharged into the pond studied.
In spite of the potential sources of many types of chemicals related to the agricultural practices around this pond, the levels of all elements in water and fish samples analyzed were not violated the maximum permissible levels established by WHO and US EPA regarding water used for fish cultured pond at (100, <1.1, 100, 2000, <100, <3.2, 5000) μg/l for As, Cd, Cr, Cu, Ni, Pb and Zn as well as for freshwater fish muscles at (1.0, 30, 2.0, 0.5-1.0, 100) μg/g wet wt. for Cd, Cu, Ni, Pb and Zn, respectively [29-31]. No data available for the maximum acceptable levels of As and Cr in fish. In a previous study in Egypt, the high levels of zinc, iron, copper, cadmium and lead were found in fish tissues samples collected from the Nile river tributaries and drainage canals averages of (24.35, 108.26, 1.495, 1.840 and 1.864) μg/g, respectively. Lead and cadmium were quantified at higher concentrations than those recommended by FAO for fish .
Polycyclic aromatic hydrocarbons
Figure 4 illustrates the detected levels of the tested polycyclic aromatic hydrocarbons (PAHs) in water of fish aquaculture pond localized between vegetable and fruit fields, June-November, 2017. Naphthalene, acenaphthene, phenanthrene, fluorine, anthracene, fluoranthene, benzo(a)anthracene and benzo(b)fluoranthene were detected and ranged between 0.05 μg/l and 0.71 μg/l. The other tested PAHs; Pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, benzo(g,h,i)perylene, indeno(1,2,3-cd) pyrene and dibenzo(a,h)anthracene were below the method detection limits (0.15 μg/l-0.45 μg/l) at all months during the sampling period. Residual levels of PAHs were also determined in Nile Tilapia and grey Mullet fish muscles of the aquaculture pond. Figure 5 shows that some of PAHs tested namely; Phenanthrene, fluorene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, benzo(b)fluoranthene, and dibenzo(a,h)anthracene were detected at a range of 0.18 μg/g-0.72 μg/g fresh wt. fish muscles, with no remarkable difference for the PAH levels between the two types of fish. Naphthalene, acenaphthylene, acenaphthene, benzo(k)fluoranthene, benzo(a)pyrene, benzo(g,h,i)perylene and indeno(123-cd) pyrene were below the detection limits (<DLs) which ranged from 0.65 μg/g to 5.53 μg/g fresh wt.). Generally, medium molecular weight PAHs with 3-5 benzene rings were predominant in the two types of fish compared with those in water samples analyzed (3-4 rings). The obtained levels of PAHs in all water analyzed were below the water quality guidelines  which specify the permissible accepted levels for naphthalene, acenaphthene, phenanthrene, fluorine, fluoranthene and benzo(a)anthracene in fresh water at (1, 6, 0.3, 12, 4 and 0.1) μg/l, respectively. It must also be noted that although benzo(a)pyrene was below the method detection limit in this study, the European Union determined only the permissible limit of benzo(a)pyrene (0.002 ppm in fish) due to its serious public health significance among all the PAHs compounds . A previous research was carried out on Tilapia and Mullet fish samples collected from different markets at Sharkia Governorate, Egypt. Eleven PAHs namely; acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, chrysene, benzo(b)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene and benzo(g,h,i)perylene were detected in the fish tissues. No significant difference between the mean total PAHs residues in the two examined fish species. Benzo (a) pyrene levels were exceeded the permissible limits in 15% and 55% of Tilapia and Mullet samples, respectively .
Figure 6 shows the detected levels of organochlorine, organophosphorus and pyrethroid pesticides in water of fish aquaculture pond surrounded by vegetable and fruit fields, June- November, 2017. Trace levels of α-HCH, ϒ-HCH, endosulfan ɪ, endosulfan ɪɪ, endosulfan sulfate, heptachlor, heptachlor epoxide, p,p´- DDE, chlorpyrifos, cypermethrin and cyfluthrin were detected in water with the averages of (0.77-0.114, 1.15-1.26, 0.78-1.31, 0.80-1.44, 0.15-1.04, 0.91-1.24, 0.84-1.53, 0.60-1.44, 0.12-1.03, 0.26-1.04 and 0.11-0.23) ng/l, respectively. The same compounds were also detected in the two types of fish analyzed (except endosulfan sulfate, cyfluthrin and fenvalerate below the minimum detection limits (3.7 ng/g-6.5 ng/g fresh wt.)) at ranges of 0.87 ng/g-10.21 ng/g fresh wt. of Tilapia and 0.95 ng/g-8.32 ng/g fresh wt. of Mullet muscles (Figure 7). p,p´-DDE was a predominate residue quantified in the two types of fish.
The organochlorines in fish tissues of El Sharkia and El Menofiya governorates, Egypt were mostly detected in the safe limits admitted by FAO and WHO except for chlordane [7,34]. In the present study, the occurrence of organophosphorus (chlorpyrifos) and pyrethroid (cypermethrin, cyfluthrin and fenvalerate) insecticides mainly attributed to its intensive current application during the growing and fruiting periods of vegetables and fruits around the fish aquaculture pond investigated, while the occurrence of the other detected pesticide residues (organochlorine compounds) not due to its direct application on crops but expected to reach the water and fish studied as environmental contaminants transported and accumulated via different food chains same as the other persistent organic pollutants (POPs). Residue levels of these pollutants and their bioaccumulation have been intensively monitored by many of other researchers. For example in the ecosystem of lake Temsah ecosystem, Ismailia. The OCP levels reported in biota were below FDA regulatory action levels of 0.3 μg/g. For biota, bioaccumulation factors (BAF) of OCPs were at 23.7-560 much higher than those of PAHs (0.1-1.2), with heptachlor epoxide showing the highest mean BAF . Another study on the distribution pattern of certain POPs was evaluated in water, sediment and aquatic biota of O. niloticus and Donax trunculus at the Rosetta Nile branch estuary. The results found that α-HCH, p,p′-DDE and PCBs were the predominant compounds detected at ranges of 0.54 ng/ g-4.90 ng/l water, 0.75 ng/g-2.41 ng/g, d. wt. sediment and 2.19 ng/ g-28.11 ng/g, fresh wt. biota, whereas β and γ-HCHs, endosulfan compounds, heptachlor and heptachlor epoxide detected at low frequencies. The total POPs were found at high levels and abundances in Donax spp. than in Tilapia spp., and both at below its tolerable residue levels .
Based on the data listed in Table 2 concerning freshwater quality criteria for the acute and chronic levels of organochlorine, organophosphorus and pyrethroid pesticides, the obtained levels of the tested pesticides in water and fish tissues of the pond investigated were within the permissible limits, as well as, within the acceptable daily intake (ADI) levels of pesticide residues established by the FAO/WHO, and consequently, below its tolerable average residue levels (TARLs) in muscle tissues of Nile Tilapia and grey Mullet fish analyzed were .
|Pesticides||(a) Freshwater-quality criteria (ng/l)||Acceptable daily intake in fish (mg/kg bw/day)|
|Endosulfan ɪ||220||5.6||(c) 6.0|
(a) EPA ambient freshwater-quality criteria of acute and chronic levels for aquatic organisms.
(b) N.A: Not available.
(c) Sum of endosulfans.
(d) Sum of DDTs.
Table 2: Freshwater and fish quality criteria for the tested pesticides.
In spite of some physicochemical parameters measured at levels violated the water quality guidelines for the management of pond fish culture, it can negatively affect the fish growth and quality of such aquaculture ponds. Fortunately, the Tilapia and for somewhat Mullet fish are easy to tolerate a wide range of environmental conditions [36,37]. At the level of human health, the study recommends that regular monitoring is therefore required to control the levels of different environmental contaminants such as PAHs and pesticide residues in different aquatic ecosystem including fish aquaculture ponds. Also, the private aquaculture fish ponds must be subjected to good aquaculture practices supervised by the responsible food safety authority.
The author thanks the neighbour farmers for their help during the field sampling of this study. This research did not receive any specific grant from funding agencies in the public, commercial, or not-forprofit sectors, and there is no conflict of interest identified.
- FAO (2018) National Aquaculture Sector Overview, Egypt. Fisheries and Aquaculture Department, Food and Agriculture organization of the United Nations, Rome, Italy.
- General Authority for Fish Resources Development (2010) Statistics of fish production of year 2009. Ministry of Agriculture and Land Reclamation. Cairo, Egypt pp: 104.
- USDA Foreign agricultural services, global agricultural information network (2016) The State and Development of Aquaculture in Egypt. Cairo, Egypt.
- Feidi I (2018) Will the new large-scale aquaculture projects make Egypt self-sufficient in fish supplies ? MedFAR 1: 31-41.
- Malhat F (2011) Distribution of heavy metal residues in fish from the River Nile tributaries in Egypt. Bull Environ Contam Toxicol 87: 163-165.
- Emara MM, Farag RS, Dawah A, Fathi M (2015) Assessment of Heavy Metals Concentration in Water and Edible Tissues of Nile Tilapia (Oreochromis niloticus) from two Fish Farms Irrigated with Different Water Sources, Egypt. International Journal of Environment 04: 108-115.
- Malhat F, Nasr I (2011) Organophosphorus pesticides residues in fish samples from the River Nile tributaries in Egypt. Bull Environ Contam Toxicol 87: 689-692.
- Nasr IN, EL-Enaen NHA, Yosef TA (2012) Study of Some Polycyclic Aromatic Hydrocarbons Residues in Fish at Sharkia Governorate Markets in Relation to Public Health. Global Veterinaria 8: 670-675.
- Almatari MS, Ahmed YM, Reda L, Naglaa L, Ahmed TM (2017) Residues of some organic pollutants, their bioaccumulation, and risk assessments profile in Lake Temsah, Ismailia, Egypt. J Clin Exp Tox 1: 7-20.
- Abbassy MMS (2018) Distribution pattern of persistent organic pollutants in aquatic ecosystem at the Rosetta Nile branch estuary into the Mediterranean Sea, north of delta, Egypt. Marine Pollution Bulletin 131: 115-121.
- Mohamed AG, El Safty AM, Siha MS (2013) Current situation of water pollution and its effect on aquatic life in Egypt. Egyptian Journal of Occupational Medicine 37: 95-119.
- Anonymous (2002) Project for an intensive fish farm El-Gouna Aquaculture Pilot Project (GAPP). Technical report submitted to ORASCOM Company, El-Gouna resort, Red Sea Governorate, Egypt. Cairo, Aquaculture Consultant Office pp: 7
- APHA (1995) Standard methods for the examination of water and waste water, 10th ed. Washington: American Public Health Association 391-448.
- USEPA Method 1801 (1993) Determination of Turbidity by Nephelometry. Environmental monitoring systems laboratory office research and development. USEPA. Cincinnati, OHIO 45268.
- USEPA Method 1664 (2010) Revision B: n-Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n-Hexane Extractable Material by Extraction and Gravimetry.
- USEPA Method 2007 (1994) Determination of Metals and Trace Elements in Water and Wastes by Inductively Coupled Plasma-Atomic Emission Spectrometry.
- USEPA Method 6010C (2000) Inductively Coupled Plasma-Atomic Emission Spectrometry.
- USEPA Method 610 (1984) Methods for organic chemical analysis of municipal and industrial wastewater: Polynuclear Aromatic Hydrocarbons.
- USEPA Method 608 (1984) Organochlorine Pesticides and PCBs. EPA 600/4-84-061, National Technical Information Service PB84-211358 Springfield Virginia 22161.
- Yunker MB, Macdonald RW, Vingarzan R, Mitchell RH, Goyette D, et. al. (2002) PAHs in the Fraser River Basin: a critical appraisal of PAH ratios as indicators of PAH source and composition. Organic Geochem 33: 489-515.
- Ekpo BO, Fubara, EP, Ekpa OD, Marynowski HL (2012) Determination of hydrocarbon sources using n-alkane and PAH distribution indices in sediments from coastal areas of Bonny river in Niger Delta, Nigeria. Asian Res Pub Network (ARPN) 1: 9-20.
- Amaraneni SR, Pillala RR (2001) Concentration of pesticide residues in tissues of fish from Kolleru Lake in India. Environ Toxicol 16: 550-556.
- Bhuvaneshwari R, Rajendran RB (2012) GCMS determination of Organochlorine Pesticides (OCPs) in Fish from River Cauvery and Veeranam Lake. E-Journal of Chemistry 9: 2346-2353.
- Abbassy MMS (2017) Pesticide residues in Buffalo and Human Breast Milk of Vegetables and Fruits Farming Community at Northern of Delta in Egypt. J Environ Anal Toxicol 7: 1-6.
- Fillmann G, Readman JW, Tolosa I, Bartocci J, Villeneuve JP, et al. (2002) Persistent organochlorine residues in sediments from the Black Sea. Mar Poll Bull 44: 122-133.
- Svobodova Z, Lloyd R, Machova J, Vykusova B (1993) Water Quality and Fish Health. Rome: FAO pp: 54.
- Lawson TB (1995) Fundamentals of Aquacultural Engineering. New York: Chapman and Hall.
- Bhatnagar A, Devi P (2013) Water quality guidelines for the management of pond fish culture International Journal of Environmental Sciences 3: 1980-2009.
- WHO (1993) Evaluation of Certain Food Additives and Contaminants. World Health Organization, Geneva pp: 837.
- US EPA (1986) Quality Criteria for Water 1986. EPA 440/5-86-001. Washington, DC: United States Environmental Protection Agency (US EPA); Office of Water, Regulations and Standards.
- WHO (1989) Health Guidelines for the Use of Wastewater in Agriculture and Aquaculture. Report of a Scientific Group. World Health Organization: Geneva pp: 778.
- Anzecc, Armcanz (2000) Australian guidelines for water quality monitoring and reporting. National Water Quality Management Strategy, No. 7, Australian and New Zealand Environment and Conservation Council & Agriculture and Resource Management Council of Australia and New Zealand, Canberra.
- Official Journal of the European Union (2006) Levels for certain contaminants in foodstuffs. Section 10. 6: Polycyclic Aromatic Hydrocarbons: 6.1.2.
- Mohamed AA, Tharwat AE, Khalifa HA (2016) Estimation of organochlorine pesticides and heavy metal residues in two species of mostly consumed fish in Sharkia Governorate, Egypt. Japanese Journal of Veterinary Research 64: 233-241.
- FAO/WHO (2014) Codex pesticides residues in food online database.
- Nowell LH, Resek EA (1994) National standards and guidelines for pesticides in water, sediment, and aquatic organisms: application to water-quality assessments. Rev Environ Contam Toxicol 140: 1-164.
- Grammer GL, Slack WT, Peterson MS, Dugo MA (2012) Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) establishment in temperate Mississippi, USA: multi-year survival confirmed by otolith ages. Aquatic Invasions 7: 367-376.
Citation: Abbassy MMS (2018) Water and Fish Quality of Aquaculture Pond Adjacent to Intensive Pesticides Application Agro-System. Fish Aqua J 9: 251. DOI: 10.4172/2150-3508.1000251
Copyright: © 2018 Abbassy MMS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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