alexa 16S-23S Intergenic Spacer (ITS) Region Sequence Analysi
ISSN: 2327-5073

Clinical Microbiology: Open Access
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Research Article

16S-23S Intergenic Spacer (ITS) Region Sequence Analysis: Applicability and Usefulness in Identifying Genera and Species Resembling Non-Hemolytic Streptococci

Xiaohui Chen Nielsen1*, Derya Carkaci1, Rimtas Dargis1, Lise Hannecke1, Ulrik Stenz Justesen2, Michael Kemp2, Jens Jørgen Christensen1 and Monja Hammer3

1Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark

2Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark

3Department of Microbiology Diagnostics and Virology, Statens Serum Institute, Copenhagen, Denmark

*Corresponding Author:
Xiaohui Chen Nielsen, MD, Ph.D
Department of Clinical Microbiology
Slagelse Hospital, Ingemannsvej 18
4200 Slagelse, Denmark
Tel: +4558559479
Fax: +4558559410
E-mail: [email protected]

Received date: August 23, 2013; Accepted date: September 24, 2013; Published date: September 28, 2013

Citation: Nielsen XC, Carkaci D, Dargis R, Hannecke L, Justesen US, et al. (2013) 16S-23S Intergenic Spacer (ITS) Region Sequence Analysis: Applicability and Usefulness in Identifying Genera and Species Resembling Non-Hemolytic Streptococci. Clin Microbial 2:130. doi: 10.4172/2327-5073.1000130

Copyright: © 2013 Nielsen XC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The species that are catalase-negative, gram-positive cocci and not belonging to streptococci or enterococci have become increasingly well characterized and the number of taxonomic entities steadily growing based on molecular taxonomic studies. This complicates their identification. 16S-23S Intergenic Spacer (ITS) region sequence analysis has proven to be a useful tool for species identification of the genera Streptococcus and Enterococcus. This study investigated the possibility of using ITS sequence analysis as a common tool for species identification within the genera Aerococcus, Abiotrophia, Alloiococcus, Dolosicoccus, Dolosigranulum, Facklamia, Granulicatella, Gemella, Ignavigranum, Leuconostoc, and Vagococcus. ITS sequences of 29 type strains and 103 well-characterized clinical strains were determined and BLAST analysis performed for species identification. All clinical strains were convincingly identified to the species level. Phylogenetic analysis showed distinct clustering of strains with the allocated species and the respective type strains. Thus, ITS sequence analysis was useful for species identification of bacteria belonging to the genera that are catalase-negative and gram-positive cocci. Potentially, ITS could be considered as the first line identification tool for the group of catalase-negative, gram-positive cocci, including nonhemolytic streptococci, enterococci and the taxons examined in this study.

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