2-0, 3-0 Desulfated Heparin does not Affect Radiation Injury Induced Mortality but Reduces Radiation Combined Skin-burn Injury Induced Survival in Mice
- *Corresponding Author:
- Aminul Islam
Ph.D. Armed Forces Radiobiology Research Institute
Uniformed Services University of the Health Sciences
8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA
E-mail: [email protected]
Received Date: April 22, 2015 Accepted Date: June 15, 2015 Published Date: June 18, 2015
Citation: Islam A, Bolduc DL, Zhai M, Hobbs SS, Swift JM (2015) 2-0, 3-0 Desulfated Heparin does not Affect Radiation Injury Induced Mortality but Reduces Radiation Combined Skin-burn Injury Induced Survival in Mice. J Cell Sci Ther 6:209. doi:10.4172/2157-7013.1000209
Copyright: © 2015 Islam A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Many radiation events have involved a high incidence of radiation combined injuries. Victims of radiation events succumb to serious infections as a consequence of bacterial translocation and sepsis. Exacerbation of the risk of infection by radiation combined burn injury (RCBI) further heightens vulnerability. There are currently no suitable countermeasures that exist for RCBIs. We evaluated 2-0, 3-0 desulfated heparin (ODSH), an anti-inflammatory and anticoagulant agent as a potential countermeasure to RCBI. Female B6D2F1/J mice (12-week) were subjected to 9.5 Gy (LD70/30 for RCBI) whole-body bilateral 60Co gamma-photon radiation (0.4 Gy/min), followed by dorsal skin burn injury under anesthesia (∼15% total-body-surface area burn). Mice were injected subcutaneously with ODSH (25 mg/kg every 12 h; days 1-2 and 17.5 mg/kg every 12 h; days 3-7) or vehicle (sterile saline of equal volume) for 7 days post-injury and further administered topical gentamicin (0.1% cream; days 1-10) and oral levofloxacin (100 mg/kg; days 3-16). Mice were euthanized on day 30 following water consumption, body mass and survival analysis. Our data showed ODSH had no effect on radiation injury (RI)-induced mortality (45% ODSH vs. 45% VEH; n=20). However interestingly, ODSH treatment significantly reduced survival after RCBI (12% ODSH vs. 41% VEH; n=22, p<0.05). Furthermore, ODSH did not affect water consumption or body mass accrual after RI or RCBI. ODSH was not able to counteract the negative alterations in hematology, splenocytes, or bone marrow cell counts after RI or RCBI. These data illustrate that ODSH in combination with antibiotic treatments, may not be a mitigating countermeasure for RCBI.