A Bicistronic DNA vaccine against Porcine Circovirus and Porcine ParvovirusWencheng Lin#, Zhenmei Zhang# and Shangjin Cui*
Division of Swine infectious Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin 150001, China
- *Corresponding Author:
- Dr. Shangjin Cui, DVM, PhD
Division of Swine infectious Disease
State Key Laboratory of Veterinary Biotechnology
Harbin Veterinary Research Institute
No. 427, Maduanjie, Nangang District
Harbin, Heilongjiang 150001
People's Republic of China
Tel: 86 451 85935093
Fax: 86 451 82733132
E mail: [email protected]
Received date: August 12, 2011; Accepted date: September 21, 2011; Published date: September 28, 2011
Citation: Lin W, Zhang Z, Cui S (2011) A Bicistronic DNA vaccine against Porcine Circovirus and Porcine Parvovirus. J Veterinar Sci Technol S1:001. doi:10.4172/2157-7579.S1-001
Copyright: © 2011 Lin W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Porcine parvovirus (PPV) is a widespread, infectious virus associated with reproductive disease of swine and death of piglets, and porcine circovirus type 2 (PCV2) is an important emerging pathogen associated with a number of different syndromes and diseases in pigs.
Objectives: The aim of this study was to construct a series of recombinant plasmids used for immunizing mice, and measure the immune responses.
Methods: A bicistronic DNA vaccine against PPV and PCV2 infection was developed by subcloning PPV-VP2 and PCV2-ORF2 genes into a bicistronic vector. After the in vitro expression of both the proteins was characterized, the bicistronic DNA vaccine and monocistronic DNA vaccines were injected into mice and the induced immune responses were compared with monocistronic DNA vaccines.
Results: There was no significant difference in ELISA antibody, virus neutralizing antibody and cellular immune responses against PPV and PCV2 in mice immunized with bicistronic or monocistronic DNA vaccine, respectively.
Conclusions: This study indicates that bicistronic DNA vaccine can induce humoral and cellular immune responses in mice against both PPV and PCV2.