A Bovine Collagen Type I-Based Biodegradable Matrix as a Carrier for Tissue-Engineered UrotheliumMartin Vaegler1#, Lisa Daum1#, Sabine Maurer1, Arnulf Stenzl2, Silke Busch3 and Karl-Dietrich Sievert1,2,4*
- *Corresponding Author:
- Karl-Dietrich Sievert
MD PhD, FACS, FRCS, Professor and
Chair of Department of Urology and
Andrology, Uro-Oncology, Neurourology
Incontinence and Reconstructive Urology
Paracelsus Medical University
Strubergasse 21, 5020 Salzburg, Austria
Tel: +43-662 448 229 51
Fax: +43-662 448 229 94
Email: [email protected]
Received date: March 10, 2015; Accepted date: April 07, 2015; Published date: April 09, 2015
Citation: Vaegler M, Daum L, Maurer S, Stenzl A, Busch S, et al. (2015) A Bovine Collagen Type I-Based Biodegradable Matrix as a Carrier for Tissue-Engineered Urothelium. J Stem Cell Res Ther 5:275. doi:10.4172/2157-7633.1000275
Copyright: © 2015 Vaegler M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Engineering of lower urinary tract tissue suitable for reconstructive surgery requires biomaterials as cell carriers, particularly in patients for whom autologous grafts are not available. Matrices should support growth, improve mechanical stability, feature excellent biocompatibility, and fully degrade without signs of scarring at the implantation side. In this study, a new bovine collagen type I-based biodegradable non-cross linked matrix was investigated for its suitability as a carrier for porcine and human urothelial cells in vitro. Initial cell adherence, metabolic activity, and proliferation behaviour of cells isolated from tissue biopsies were analyzed. Constructs were characterized immunohistologically in comparison with matrix-free cell sheets established on plastic surface (=controls). Even for high-density seeding, adherence on collagen cell carrier (CCC) was excellent. Metabolic activity and proliferation of stratifying porcine and human urothelial cells cultured on CCC were comparable to that of controls. Immunofluorescence analysis confirmed epithelial phenotype, cell-cell junction formation and ongoing differentiation of the multilayered urothelium on CCC. This study proved CCC as a suitable carrier for urothelial cells for the future aim for urethral reconstruction.