A Comparative Antimicrobial activity of the Schinus Terebinthifolius obtained by Micropropagation and Outdoor Grown
Schinus terebinthifolius is widely used in popular medicine, especially as an anti-inflammatory, an antiseptic and an antimicrobial, due to that, it is commonly chosen in biological, clinical, pharmacological and chemical studies. The aim of this work was to evaluate the antimicrobial activity in of the S. terebinthifolius extracts obtained from outdoor-grown specimen and micro propagated ones in aseptic conditions (in vitro) and to study the influence of entophytic fungi in the productions of plant metabolites having antimicrobial activity by plant. Micro propagated extracts and outdoor-grown S. terebinthifolius plants were evaluated for their antibacterial and antifungal activities tested against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans using the agar diffusion and the micro dilution methods. Phytochemical compounds were determined using HPLC. The extracts from micro propagated and outdoor-grown plants showed good antibacterial activity against the tested bacterial strains. The extracts from micro propagated plants showed the best antibacterial activity with a minimum inhibitory concentration (MIC) of 0.2 mg/mL against S. aureus. The results form a good basis for the use of S. terebinthifolius micro propagated plants as a complement to the use of outdoor-grown plants in traditional medicine, moreover this study has concluded that the endophytic fungi seem not to stimulate the plant production of phenolic compounds against the pathogenic strains used in this study.