alexa A Comparative Study of New Castle Disease Virus Isolated From Poultry with Standard Velogenic Strain | OMICS International
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

A Comparative Study of New Castle Disease Virus Isolated From Poultry with Standard Velogenic Strain

Karmakar DB1*, Majee SB2, Karikkathil SS3, Seth D4 and Mondal S5

1Block Animal Health Center, Contai-III, Purba Medinipur, India

2Department of Microbiology, Bombay Veterinary College, Parel, Mumbai-400012, India

3Krishi Vigyan Kendra Ernakulam, Narakkal-post, Ernakulam district, Kerala-682 505, India

4Block Animal Health Center, Sitai, Coachbihar, W.B, India

5Additional Block Animal Health Center, Balarampur, Puruila, W.B, India

*Corresponding Author:
Dibyendu Bikash Karmakar
C/O Kanai Seth, Vill-Seth Colony
PO+PS-Kaliyaganj, Dist-Uttar Dinajpur, 733129, India
Tel: +919475724596
E-mail: [email protected], [email protected]

Received date: April 01, 2015; Accepted date: April 23, 2015; Published date: April 25, 2015

Citation: Karmakar DB, Majee SB, Karikkathil SS, Seth D, Mondal S (2015) A Comparative Study of New Castle Disease Virus Isolated From Poultry with Standard Velogenic Strain. J Veterinar Sci Technol 6:231. doi:10.4172/2157-7579.1000231

Copyright: © 2015 Karmakar DB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Newcastle disease [avian paramyxovirus serotype 1 (APMV-1)] is highly prevalent in poultry thought India. The present study was undertaken to extrapolate the pathogenecity of a field isolate of Newcastle disease virus (NDV) in comparison with velogenic BOM-1 isolate (AY 294125). The field NDV isolate and the BOM-1 isolate were propagated in SPF eggs and the infected allantoic fluids were used for characterization of pathotype. The Mean Death Time (46 hours), Intracerebral Pathogenicity Index (1.74), Intravenous pathogenicity Index (2.01) were enumerated to find out the pathotype of the virus. The field NDV isolate was able to agglutinate mammalian erythroytes except equine RBCs, stable at 56°C for 60 mins and proved to be a rapid eluter. It formed plaques (5.39 x 106 PFU/ ml) in chick embryo fibroblast with characteristic cytopathic effects of velogenic NDV comparable with BOM-1 isolate. The viral proteins that were detected by SDS-PAGE were HN (76 KD), F (66 KD), NP (55 KD) and M (44 KD). The total protein in the field virus isolate was determined to be 18.75 mg%, which was lower than that of the total proteins in BOM-1 isolate (45 mg%). The field virus contained 28.83 mg% of total lipids and 4.98 mg% total carbohydrates, which were more or less similar to that of BOM-1 isolate and that of allantoic fluid. On the basis of all the biological and the chemical tests conducted, the field virus isolate included in this present study was characterized as velogenic viscerotropic strain of NDV.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version