A Comparison Study of Histochemical Staining of Various Tissues after Carnoy's Versus after Formalin FixationHussain Gadelkarim Ahmed* and Ahmed Ibn idris Mohammed
Department of Histopathology and Cytology, Faculty of Medical laboratory Sciences, University of Khartoum, Khartoum, Sudan
- *Corresponding Author:
- Dr. Hussain Gadelkarim Ahmed
University of University of Khartoum
102, Faculty of medical laboratory sciences
Tel: +249 155 130 423
E-mail: [email protected]
Received date: January 29, 2011; Accepted date: March 02, 2011; Published date: March 09, 2011
Citation: Ahmed HG, idris Mohammed AI (2011) A Comparison Study of Histochemical Staining of Various Tissues after Carnoy's Versus after Formalin Fixation . J Cancer Sci Ther 3: 084-087. doi: 10.4172/1948-5956.1000065
Copyright: © 2011 Ahmed HG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: The potential problems related to the use of formalin in histopathology, such as health hazards, deterioration of nucleic acids are well-known. The aim of this study was to evaluate the utilization of a Carnoy's solution fixation in comparison with formalin on subsequent tissue sectioning and histochemical staining. Materials and Methods: Corresponding sections of 25 tissue biopsies of rabbit's different organs were fixed in Carnoy's solution and in 10% neutral buffered formalin. Samples were processed using the conventional method and then stained applying five histochemical methods. The degree of the quality of the staining was assessed for each method by scoring system (1-10) depending on comparison of the stained tissue sections with illustrated photomicrographs. Results: For the quality of cutting, the best quality was obtained by Formalin (mean = 4.76) then Carnoy's fixative (mean =3.84). The best quality of Haematoxylin and Eosin staining was obtained by formalin (mean =5.28) then Carnoy's (mean = 4.00). For Alcian blue and Perl's Prussian blue, the best staining qualities were obtained by Formalin (mean = 4.76 and 5.64 respectively) followed by Carnoy's (mean = 2.88 and 3.92 respectively). For periodic Acid Schiff's the best staining quality was obtained following Carnoy's fixation (mean = 4.52) then, the formalin (mean = 3.76). Conclusion: Although, Carnoy's fluid is a save fixative and can rapidly penetrate the tissues, but it can't be a substitute for formalin.