alexa A Genetic Fusion between Sm14 and CTB does not Reduce Schistosoma mansoni Worm Burden on Intranasally Immunized BALB/c Mice
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

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Research Article

A Genetic Fusion between Sm14 and CTB does not Reduce Schistosoma mansoni Worm Burden on Intranasally Immunized BALB/c Mice

Henrique Roman Ramos1,2, Patricia Aoki Miyasato3, Celso Raul Romero Ramos4, Ana Paula de Mattos Arêas5, Toshie Kawano3 and Paulo Lee Ho1,2*

1Centro de Biotecnologia, Instituto Butantan. Av. Vital Brasil, 1500 São Paulo SP, Brazil 05503-900

2Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo. Av. Prof. Lineu Prestes, 748 São Paulo SP, Brazil 05513-970

3Laboratório de Parasitologia, Instituto Butantan. Av. Vital Brasil, 1500 São Paulo SP, Brazil 05503-900

4Departamento de Helmintologia, FIOCRUZ. Av. Brasil, 4635 Rio de Janeiro RJ, Brasil 21040-900

5Universidade Federal do ABC. Rua Santa Adélia, 166 Santo André SP, Brasil 09210-170

*Corresponding Author:
Dr. Paulo Lee Ho
Laboratório de Biotecnologia Molecular I
Centro de Biotecnologia, Instituto Butantan. Av. Vital Brasil
1500 São Paulo SP, Brazil 05503-900
Tel: +55 11 3726-7222 ext. 2244
Fax: +55 11 3726-1505
E-mail: [email protected]

Received Date: December 17, 2010; Accepted Date: December 29, 2010; Published Date: December 30, 2010

Citation: Ramos HR, Miyasato PA, Ramos CRR, de Mattos Arêas AP, Kawano T, et al. (2010) A Genetic Fusion between Sm14 and CTB does not Reduce Schistosoma mansoni Worm Burden on Intranasally Immunized BALB/c Mice. J Vaccines Vaccin 1: 111. doi: 10.4172/2157-7560.1000111

Copyright: © 2010 Ramos HR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Developing a vaccine against schistosomiasis would be an important advance on the control of this chronic and debilitating disease that afflicts millions of people worldwide. Herein we describe the use of the non-toxic B subunit of cholera toxin (CTB) genetically fused to Sm14 - a fatty-acid binding protein from Schistosoma mansoni - as an attempt to elicit a mucosal immune response against the lung stage of this parasite by intranasal immunization. Recombinant proteins were expressed on a prokaryotic system, purified by affinity chromatography and both immunochemically and spectroscopically characterized. Intranasal immunization experiments were performed on BALB/c mice and vaccine efficacy was assessed analyzing the worm-burden after challenge infection with S. mansoni cercariae. The results demonstrate that Sm14 itself was not able to reduce the worm burden on intranasally vaccinated animals. The presence of CTB – either in intranasal coadministration with or genetically fused to Sm14 – did not significantly improve the protective response of Sm14 as a worm burden reduction of only 20% could be observed. In addition to that, however, CTB demonstrated a clear anti inflammatory effect on the liver of immunized mice, which displayed hepatic granulomas around trapped eggs 15% smaller than control groups, indicating that CTB displays an immunomodulatory effect on the inflammatory responses induced by the parasite egg toxins.

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