alexa A High Sensitive Nested PCR for Toxoplasma gondii Detection in Animal and Food Samples
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Short Communication

A High Sensitive Nested PCR for Toxoplasma gondii Detection in Animal and Food Samples

Maria Vitale1*, Paola Galluzzo1, Vittoria Currò1, Kataryna Gozdzik2, Domenico Schillaci3 and Vincenzo Di Marco Lo Presti1

1Zooprofilactic Experimental Institute of Sicily (IZS Sicily), A. Mirri, via Gino Marinuzzi 3, Palermo 90129, Italy

2Witold Stefanski Institute of Parasitology of the Polish Academy of Sciences, 51/55 Twarda Street-00818, Warsaw, Poland

3Department of Biological, Chemical and Pharmaceutical Science and Technology (STEBICEF), University of Palermo, via Archirafi 32, Palermo 90123, Italy

*Corresponding Author:
Maria Vitale
Zooprofilactic Experimental Institute of Sicily (IZS Sicily)
A. Mirri, via Gino Marinuzzi 3, Palermo 90129, Italy
E-mail: [email protected]

Received date: February 19, 2013; Accepted date: March 14, 2013; Published date: March 18, 2013

Citation: Vitale M, Galluzzo P, Currò V, Gozdzik K, Schillaci D, et al. (2013) A High Sensitive Nested PCR for Toxoplasma gondii Detection in Animal and Food Samples. J Microb Biochem Technol 5:039-041. doi:10.4172/1948-5948.1000097

Copyright: © 2013 Vitale M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Toxoplasma gondii is a major food and waterborne transmitted parasite world-wide. The tissues and meat samples of many warm blooded animals can contain tissues cysts from chronic toxoplasmosis. Water and vegetable can be contaminated by the parasitic oocysts shed through the feces of infected cats, representing the definitive host of the parasite.

A sensitive PCR for Toxoplasma gondii detection is described. The first step amplified the region between the 28S and 18S rDNA in the closely related T. gondii and Neospora caninum; RFLP analysis distinguished the DNA from the two morphologically identical parasites. Although N. caninum is not involved in human transmission, so far, it is important for animal health since is a major responsible for abortion in cattle.

The nested PCR was used in a dilution assay in pork sausage samples spiked with T. gondii parasitic DNA. The analysis showed that up to 200fg equivalent to two single parasites only, could be detected. Similar detection limit for T. gondii can be obtained with real-time PCRs, but real time methods need special consumables and expensive equipment.

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