alexa A Loop-mediated Isothermal Amplification Platform for the Detection of Foodborne Pathogens
ISSN: 2155-9538

Journal of Bioengineering & Biomedical Science
Open Access

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Research Article

A Loop-mediated Isothermal Amplification Platform for the Detection of Foodborne Pathogens

Birmpa A1, Kouroupis G2, Kalogeropoulos K1, Kokkinos P1, Kritsonis P2and Vantarakis A1*

1Environmental Microbiology Unit, Department of Public Health, Medical School, University of Patras, 26504, Greece

2Metricon Digital Systems, Athinon 65, Rio, Patras, 26441, Greece

*Corresponding Author:
Vantarakis A
Associate Professor
Environmental Microbiology Unit
Department of Public Health
School of Medicine
University of Patras, Greece
Tel/Fax: +302610969875
E-mail: [email protected]

Received Date: July 28, 2015; Accepted Date: August 21, 2015; Published Date: September 04, 2015

Citation: Birmpa A, Kouroupis G, Kalogeropoulos K, Kokkinos P, Kritsonis P, et al. (2015) A Loop-mediated Isothermal Amplification Platform for the Detection of Foodborne Pathogens. J Bioengineer & Biomedical Sci 5:164. doi:10.4172/2155-9538.1000164

Copyright: © 2015 Birmpa A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Salmonella spp., Listeria monocytogenes and viruses constitute major concern for public health. It is known that RTE foods are consumed raw, thus they are often associated with outbreaks of food poisoning. In the present study, the effectiveness of a LAMP fully automated platform able to detect foodborne pathogens in less than an hour, was evaluated. Food samples were inoculated with known pathogens, such as S. enteritidis, L. monocytogenes and hAdV 40/41. With LAMP assays pathogens can be detected shortly and without the need of sophisticated equipment. The results of the present study were then compared to those of the LAMP assays detected by a Light Cycler Roche platform which is a real-time PCR instrument and were in concordance. The developed LAMP platform that is presented in this study could become a valuable, robust, innovative, powerful, cheap and fast monitoring tool which can be extensively used for routine analysis and screening of contaminated foods by the food industry and the Public Health Authorities

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