alexa A Method for Purifying Native Transthyretin from Human
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
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Research Article

A Method for Purifying Native Transthyretin from Human Plasma

Aline G Santana1,2, Paulo C Carvalho1, Nilson IT Zanchin1 and Tatiana ACB Souza1*
1Laboratory of Proteomics and Proteins Engineering, Institute Carlos Chagas, Curitiba-PR, Brazil
2Universidade Federal do Paraná (UFPR), Curitiba-PR, Brazil
Corresponding Author : Tatiana de Arruda Campos Brasil de Souza
Instituto Carlos Chagas, ICC- FIOCRUZ-PR
Rua Prof. Algacyr Munhoz Mader, 3775
bloco C, 81350-010, Curitiba-PR, Brazil
Tel: +55 41-2104-3225
E-mail: [email protected]
Received: September 10, 2015 Accepted:September 16, 2015 Published: September 21, 2015
Citation: Santana AG, Carvalho PC, Zanchin NIT, Souza TACB (2015) A Method for Purifying Native Transthyretin from Human Plasma. Biochem Pharmacol (Los Angel) 4:187. doi:10.4173/2167-0501.1000187
Copyright: © 2015 Santana AG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Transthyretin is a homotetrameric thyroid-hormone-transporting protein that binds to the retinol binding protein thus being involved in metabolism, growth, fertility, homeostasis of the cardiovascular and central nervous system, cell differentiation, reproduction, development and maintenance the cognitive processes during aging. Currently, there are several methodologies for natively purifying TTR from plasma, serum, tears, and amyloid fibrils; however, these procedures are laborious. Herein, a low-cost and simple protocol to purify TTR from human plasma is described. It involves the separation of plasma proteins by size exclusion and DEAE chromatography. The homogeneity was assessed by SDS-PAGE and by tandem mass spectrometry using an Orbitrap-XL (Thermo, San Jose-CA).

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