alexa A Method of Central Composite Design (CCD) For Optimization of Biodiesel Production from Chlorella vulgaris | OMICS International | Abstract
ISSN:2157-7463

Journal of Petroleum & Environmental Biotechnology
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Research Article

A Method of Central Composite Design (CCD) For Optimization of Biodiesel Production from Chlorella vulgaris

Rajendran R1, Kanimozhi B1, Prabhavathi P2*,Dinesh Kumar S3, Santhanam P3, Abirami M1, Karthik Sundaram S1 and Manikandan A1
1PG and Research Department of Microbiology, PSG College of Arts and Science, Coimbatore-641014, Tamil Nadu, India
2Department of Microbiology and Biochemistry, Nadar Saraswathi College of Arts and Science, Theni-625 531, India
3Marine Planktonology and Aquaculture Lab, Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu,ndia
Corresponding Author : Prabhavathi P
Department of Microbiology and Biochemistry
Nadar Saraswathi College of Arts and Science
Theni-625 53, India
Tel: 0431-240-7072
E-mail: [email protected]
Received March 24, 2015; Accepted April 08, 2015; Published April 15, 2015
Citation: Rajendran R, Kanimozhi B, Prabhavathi P, Dinesh Kumar S, Santhanam P, et al. (2015) A Method of Central Composite Design (CCD) for Optimizationof Biodiesel Production from Chlorella vulgaris. J Pet Environ Biotechnol 6:219. doi:10.4172/2157-7463.1000219
Copyright: © 2015 Rajendran R, et al. This is an open-access article distributedunder the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The direct transesterification of the oleaginous biomass using chloroform resulted in a high biodiesel yield and FAME content than the chloroform-based extraction transesterification method and the yield was almost 100%. Hexane (89%) and petroleum ether (80%) also produced biodiesel but the yield was low when compared with chloroform. Our study showed that the biodiesel yield and FAME content of the wet biomass in the direct transesterification was significantly lower than those obtained from freeze-dried biomass suggesting that drying the algae was necessary for direct transesterification. The optimization procedures were carried out with algae: ethanol ratio, catalyst amount, reaction temperature and reaction time. The central composite design (CCD) was used to optimize the various processes. The analysis of variance (ANOVA) was also carried out and the results were found to be significant. The ρ value less than 0.0001 indicated that the model was statistically significant. The optimization procedures revealed that a yield of 95% was obtained. The 3D plot was done by keeping two components constant and other two components variable. In GC-MS, the results showed that the main components found were tetradecanoic acid ethyl ester (C14:0) 2-5%, Hexadecanoic acid ethyl ester (C16:0) 26-45%, Hexadecanoic acid ethyl ester (C16:1) 25-38%, octadecanoic acid ethyl ester (C18:0) 1-2%, oleic acid ethyl ester (C18:1) 9-13%, Eicosapentaenoic acid ethyl ester (C20:5) 1.2-5.1%. As the temperature was increased the percentage of C20:5 were decreased to 1.2% which was a good indication for fuel properties.

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