alexa A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex- Urease PCR Results with Rapid Urease Test and Histopathology
ISSN: 2327-5073

Clinical Microbiology: Open Access
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Research Article

A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex- Urease PCR Results with Rapid Urease Test and Histopathology

Sinem Oktem-Okullu1, Arzu Tiftikçi2, Murat Saruc2, Bahattin Cicek2, Eser Vardareli2, Nurdan Tozun2, Tanıl Kocagöz1, Ugur Sezerman4, Ahmet-Sinan Yavuz5 and Ayca Sayi-Yazgan3*

1Department of Medical Microbiology, School of Medicine, Acıbadem University, Istanbul, Turkey

2Department of Internal Medicine, School of Medicine, Acıbadem University, Istanbul, Turkey

3Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey

4Department of Biostatistics and Medical Informatics, School of Medicine, Acıbadem University, Istanbul, Turkey

5Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabancı University, Tuzla, Istanbul, Turkey

*Corresponding Author:
Ayca Sayi-Yazgan
Department of Molecular Biology and Genetics
Istanbul Technical University, Istanbul, Turkey
Tel: +9005325844485
Email: [email protected]

Received date: April 6, 2017; Accepted date: April 27, 2017; Published date: April 30, 2017

Citation: Okullu SO, Kocagöz T, Tiftikçi A, Yazgan AS, Tozun N, et al. (2017) A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex-Urease PCR Results with Rapid Urease Test and Histopathology. Clin Microbiol 6:279. doi:10.4172/2327-5073.1000279

Copyright: © 2017 Okullu SO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Purpose: To develop a multiplex-urease PCR assay specific for ureA and ureB genes directly from gastric biopsy specimens and compare the results of multiplex-urease PCR assay with rapid urease test (RUT) and histopathology.

Methodology: This study was conducted on 109 patients. A RUT was run; histopathological staining and multiplex urease PCR were applied to biopsy specimen to detect H. pylori. Multiplex-urease PCR, RUT and histopathology results were compared by calculating Cohen’s kappa coefficient.

Results: A multiplex-urease PCR assay specific for ureA and ureB genes was developed to detect H. pylori directly from biopsy specimens. Cohen's kappa coefficient results of histopathological staining and multiplex-urease PCR indicate a substantial agreement. There was a moderate agreement between the results of histopathological staining and RUT results. There is a fair agreement between the multiplex urease PCR and the RUT results. Furthermore, the multiplex-urease PCR can detect H. pylori in some samples that are identified as negative by the rapid urease test and histopathological staining method. Moreover, in some patient samples ureA could not be detected while ureB detected.

Conclusion: Multiplex-urease PCR assay was developed to detect ureA and ureB genes of H. pylori directly from gastric biopsy specimens. Comparison results indicated that detection rate of H. pylori with multiplex-urease PCR and histopathological staining is higher than the RUT. Moreover, it is critical to apply RUT test to biopsy specimens taken from both antrum and corpus part as in multiplex urease PCR assay. Furthermore, developing a multiplex PCR for both ureA and ureB is essential to detect active H. pylori infection.

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