alexa A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam | OMICS International | Abstract
ISSN: 2161-0940

Anatomy & Physiology: Current Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam

Kiem X Trinh1*, Pham Van Thuc2, Chu Thi My1, Long X Trinh1, Thuc Thi Minh Vu1, Bao Khanh Nguyen1, Francis Markland Jr3, Stephen Sweenson3 and Radu Minea3

1Department of Toxicology, Vietnam Institute on Toxicology (VST), Vietnam

2Department of Toxicology, Haiphong Medical and Pharmacological University, Vietnam

3Departments of Biochemistry and Molecular Biology, Keck School of Medicine of USC, CA 90033, USA

*Corresponding Author:
Kiem X Trinh
Department of Toxicology
Vietnam Institute on Toxicology, Vietnam
E-mail: [email protected]; [email protected]

Received March 28, 2016; Accepted April 12, 2016; Published April 18, 2016

Citation: Trinh KX, Thuc PV, My CT, Markland FJr, Swenson S, et al. (2016) A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam. Anat Physiol 6:213. doi:10.4172/2161-0940.1000213

Copyright: © 2016 Trinh KX, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Aim: Vietnam is a tropical and agricultural country. Annually, there are 30,000 cases of snake bite. Two venomous snake families cause the big medical problem. In this, Calloselasma Rhodostoma (CR) is the most dangerous snake. Therefore, since 2001, the scientific research collaborations between Vietnam (VN) and University of Southern California (USC) were established. Since 2014 this project has been approved by VN government. The aims of the 1st project are to establish the technological process for purification of dsintegrin from CR venom of VN (CRd.VN), to determine the molecular weight, structure and its biological antitumor activities.

Methods: The process of collection, lyophilisation of CR venom from VN. Protein concentration of CR venom was determined by BCA assay. High Performance Liquid Chromatography (HPLC), SDS-PAGE, Mass spectrometry (MS) analysis and sequencing by tryptic digestion were used for purification of CRd.VN and its molecular weight (MW) and structure. Standard cell biology methods were employed to characterize CRd’s abilities (in vitro) to inhibit platelet aggregation, adhesion, migration and invasion of tumor cells. Its anti-cancer activity in a breast cancer (BC) murine model (in vivo) was tested.

Results: Peak 7 of HPLC (CRd.VN), showed a single ~10 kDa band on SDS-PAGE gel. CRd.VN’s MW, structure and the sequence are 7.33 kDa, a monomer containing 68 amino acids with an RGD motif (position 49-51) and 6 disulfide bonds. The anticancer activities of CRd.VN are very strong.

Conclusion: We have shown that CRd.VN is a possible anti-tumor agent with clinical potential. However, further research is required on CRd recombinant production, preliminary pharmacokinetics/ toxicology properties and antitumor activities.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7