A Novel Application of Wutou (Aconitum carmichaeli) and Banxia (Pinellia ternat) Aqueous Extract on Wound Healing of Rats
|Xia Xichao1, Liu Hongyang2, Wang Weina1, Liu Fei3, Hu Qingfu1, Liang Guina1, Zhang Dong1 and Liu Rongzhi1*|
|1Department of Basic Medicine, Nanyang Medical College, Nanyang, 473041, Henan Province, China|
|2Department of Plant Pathology, Nanyang Agriculture School, Nanyang, 473002, Henan Province, China|
|3Department of Clinical Medicine, Nanyang Medical College, Nanyang, 473041, Henan Province, China|
|Corresponding Author :||Rongzhi Liu
Department of Basic Medicine
Nanyang Medical College, Nanyang, China
E-mail: [email protected]
|Received May 01, 2014; Accepted May 22, 2014; Published June 02, 2014|
|Citation: Xichao X, Hongyang L, Weina W, Fei L, Qingfu H, et al. (2014) A Novel Application of Wutou (Aconitum carmichaeli) and Banxia (Pinellia ternat) Aqueous Extract on Wound Healing of Rats. Biochem Pharmacol 3:140. doi:10.4172/2167-0501.1000140|
|Copyright: © 2014 Xichao X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Objective: The purpose of current study was to investigate effects of application of Wutou and Banxia aqueous extract in the wound rats.
Methods: Rats were fulfilled a surgical lesion with a 2.0 cm resecting tissue in the dorsal fascia. Following, animals were divided into 3 groups, including model group, control group treated with 1 mg/mL of Yunnan Baiyao, and Wutoubanxia group administrated 1 mg/mL of Wutou and Banxia extract. Wound contractions in day 0, 3, 7, 11 were calculated by an image analyzer. The histological analysis was detected using hematoxilin and eosin. The levels of tumor necrosis factor α (TNF-α), interleukin-2 (IL-2), transforming growth factor-β1 (TGF-β1), and basic Fibroblast Growth Factor (bFGF) transcripts in the wound tissue were determined by real-time quantitative PCR.
Results: Compared with the control group, rats in the model group showed poor re-modeling and re-epithelization characterized by a significant decrease of neovascularization, epithelialization and fibroblast. Furthermore, the expression levels of TNF-α, IL-2 were significantly increased, and TGF-β1 and bFGF significantly decreased in the model group in contrasted with that in the control group. By contrast, the treatment of Wutoubanxia extract reversed the above-mentioned conditions caused by wound.
Conclusion: The results suggest that administration of Wutou and Banxia extract has a promoting role in wound healing of rats possibly through enhancing anti-inflammatory ability and inducing fibroblast formation.