A novel Enzyme Inhibition Assay for Screening of Type 1 Diabetes Mellitus
Islamic Azad University-Khorasgan branch, Iran
- *Corresponding Author:
- Manoochehr Messripour
Islamic Azad University-Khorasgan branch Esfahan
Esfahan IRAN (ISLAMIC REPUBLIC OF)
E-mail: [email protected]
Received Date: April 30, 2011; Accepted Date: June 20, 2011; Published Date: June 27, 2011
Citation: Messripour M (2011) A novel Enzyme Inhibition Assay for Screening of Type 1 Diabetes Mellitus. J Mol Biomark Diagn 2:107. doi:10.4172/2155- 9929.1000107
Copyright: © 2011 Messripour M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Glutamate decarboxylase (GAD) antibody has been found in patients with recent-onset insulin dependent diabetes mellitus (IDDM). Several analytical methods are described for detection and quantitation of anti-GAD antibodies whereas the inhibition of the enzyme activity by anti-enzyme antibody is given little attention. In this study a quenching fluorimetric method based on changes of fluorescence intensities upon addition antiserum into the enzyme/substrate medium is described. The activity of the purified rat brain GAD was measured by both manometeric and fluorimeteric methods in the presence of serum samples from IDDM patients (n=21) or non diabetic subjects (n=21). Significant differences between the patients and control groups were observed using both methods. However, high degree of fluorescence sensitivity of fluorimetric technique allows for less error, less time and satisfactory useful and economical for screening of IDDM in the large populations.