A Novel Molecularly Imprinted Polymer for the Selective Removal of Interfering Hemoglobin Prior to Whole Blood AnalysisTumelo Hendrick Tabane and Bareki Shima Batlokwa*
Department of Chemical and Forensic Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye, Botswana
- *Corresponding Author:
- Bareki Shima Batlokwa
Department of Chemical a nd Forensic Sciences
Botswana International University of Science and Technology
Private Bag 16, Palapye, Botswana
Tel: +267 4900117 +267 71969805
E-mail: [email protected]
Received Date: February 19, 2017; Accepted Date: March 24, 2017; Published Date: March 27, 2017
Citation: Tabane TH, Batlokwa BS (2017) A Novel Molecularly Imprinted Polymer for the Selective Removal of Interfering Hemoglobin Prior to Whole Blood Analysis. Biochem Anal Biochem 6: 315. doi: 10.4172/2161-1009.1000315
Copyright: © 2017 Tabane TH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A heavy red globular protein, hemoglobin, responsible for whole blood red pigmentation often interferes with the identification and quantification of disease associated biomarkers from whole blood, in field of molecular diagnosis. The main challenge is the direct introduction of whole blood as a sample into analyzing instruments because of its physiological complexity and `dirty` nature. For example, the red pigment in whole blood, which is characterized as ‘dirt’, usually co-elute with the biomarkers and masks them from easy chromatographic separation prior to their final detection. It also clogs the instrument’s components such as the separating columns which are known to be sensitive, hence leading to imprecise and inaccurate results during bio-assaying. To address these challenges, our lab synthesised a novel, selective, effective and a robust hemoglobin imprinted polymer, in the form of a powder, through bulk, freeradical polymerization employing molecular imprinting technology, to selectively remove interfering hemoglobin from whole blood samples prior to instrumental analysis of disease associated biomarkers. From the results, the polymer powder effectively removed hemoglobin from whole blood sample as demonstrated by the ultraviolet-visible absorbance reduction from as high as 0.794 Au to lower values of 0.193 Au before and after polymer powder application, respectively. Experimentally, the powder had a high binding ability towards the targeted hemoglobin as demonstrated by the high percentage removal efficiency of 76% from hemoglobin standard solutions, when compared to its low binding ability towards an analogous species, (chlorophyll), at 32% from chlorophyll standard solutions. Furthermore, the polymer powder proved to be robust as it removed hemoglobin interference from the ‘dirty’ complex matrix of real human whole blood samples by up to 74% hemoglobin removal which was comparable to 76% hemoglobin removal from neat standards, thus, the polymer powder demonstrated that it can work effectively in diverse environments of clean and dirty matrix. Furthermore, the polymer powder presented itself as an efficient, selective and non-destructive whole blood clean-up pre-analytical tool that with further research may replace the destructive and non-selective conventional whole blood clean-up strategies such as the commonly employed centrifugation.