A Novel Point-of-Care BioNanoSensor for Rapid HIV Detection and Treatment Monitoring
- *Corresponding Author:
- Tomasz Rozmyslowicz
Department of Pathology
University of Pennsylvania
R. 272 John Morgan Bldg
3620 Hamilton Walk
Philadelphia, PA 19104 USA
E-mail: [email protected]
Received date: March 25, 2015; Accepted date: April 28, 2015; Published date: May 08, 2015
Citation: Rozmyslowicz T, deSa J, Lec R, Gaulton GN (2015) A Novel Point-of-Care BioNanoSensor for Rapid HIV Detection and Treatment Monitoring. J AIDS Clin Res 6:454. doi:10.4172/2155-6113.1000454
Copyright: ©2015 Rozmyslowicz T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We report here a new diagnostic approach to the direct detection of HIV in blood or other body fluids that is rapid, sensitive and potentially applicable in a point-of-care setting. The approach follows on the development of a novel BioNanoSensor (BNS) device that utilizes piezoelectric technology to detect the presence of the HIV surface glycoprotein gp120 in a nanoscale format. The detection range of the BNS device for the biomarker gp120 displayed a low-end sensitivity of 6.5×104 HIV viral particles/ml, while using a small fluid sample (5 μl) and with a reaction time of less then 30 seconds. Performance of this device indicated that the BNS has utility for direct detection of HIV particles prior to, and independent from, antibody formation. Accordingly, this device holds utility to monitor the status of HIV infection both early after exposure to virus as well as during chronic HIV infection. The BNS parameters of small sample volume, compact device size, and detection sensitivity indicate that the BNS is potentially useful in the pointof- care and/or home setting for monitoring decisions regarding HIV treatment on a real-time basis.