A Novel Strategy to Identify mRNA 3Ã¢ÂÂ² UTR and mRNA Level Difference in CrohnÃ¢ÂÂs DiseaseTuo Hu1,2#, Hua-shan Liu1,2#, Chi Zhou1,2, Xian-rui Wu1,2, Yu-feng Chen1,2, Xuan-hui Liu1,2, Xiao-wen He1,2, Yi-feng Zou1,2, Xiao-sheng He1,2, Xiao-jian Wu1,2 , Ping Lan1,2 and Jia Ke1,2*#
- *Corresponding Author:
- Jia Ke
Department of Colorectal Surgery
The Sixth Affiliated Hospital
Sun Yat-sen University 26 Yuancun Erheng Rd
Guangzhou, Guangdong, PR China, 510655
Fax: 011-86-020- 38254166
E-mail: [email protected]
Received Date: December 08, 2016; Accepted Date: March 23, 2017; Published Date: March 29, 2017
Citation: Hu T, Liu H, Zhou C, Wu X, Chen Y, et al. (2017) A Novel Strategy to Identify mRNA 3′ UTR and mRNA Level Difference in Crohn’s Disease. Chemotherapy 6:226. doi: 10.4172/2167-7700.1000226
Copyright: © 2017 Hu T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract Background and Aims: Crohn’s disease (CD) is a chronic and refractory intestinal inflammatory disease. The 3′ untranslated region (3′ UTR) of mRNA transcript can regulate its own translational process post-transciptionally, and its role in CD remains unclear. The aim of this study was to identify the critical genes influencing CD phenotype via the regulation of mRNA 3′ UTR. Methods: Isoform Structural Change Model (IsoSCM) was used to evaluate the length of 3′ UTR of the whole transciptome from a RNA-seq based online CD database. Correlations between 3′ UTR length status and mRNA level were analyzed by Spearman coefficients. Correlated genes list was overlapped with published critical CD related gene list. Univariate and multivariate analysis were performed to identify the genes associated with CD phenotype. Results: Compared with normal control, 3′ UTR of 34192 genes were shortened and 57389 were lengthened among 432784 genes in CD patients. The 3′ UTR changes of 255 genes were found significantly correlated with their mRNA level. Furthermore, 8 overlapped genes were shared by above 255 correlated genes and known CD related gene list (ABCB1, FAF1, TUT2, IL27, JAK2, LTB, NAGLU and PTPN2). The mRNA level of ABCB1 and JAK2, and shortened 3′ UTR length of JAK2 transcript were found to be correlated with deep ulcer in CD patients by univariate and multivariate analysis. Conclusions: The 3′ UTR changes of CD related genes were confirmed to be related to the phenotype of CD. Our findings may provide further insight into the epigenetic mechanisms and therapeutic strategies for CD.