A Novel Technique for Harvesting Hepatic Vein Blood and Time Course of Posthepatic Reactive Oxygen Species after Liver Transplantation in Rats
- *Corresponding Author:
- Honglei Liu
Office of Nosocomial Infection
Affiliated Hospital of Hainan Medical College
31 Longhua Road, Haikou, P.R. China
E-mail: [email protected]
Received Date: February 16, 2012; Accepted Date: March 27, 2012; Published Date: April 02, 2012
Citation: Jia C, Chen Y, Liu H (2012) A Novel Technique for Harvesting Hepatic Vein Blood and Time Course of Posthepatic Reactive Oxygen Species after Liver Transplantation in Rats. J Transplant Technol Res S1: 008. doi: 10.4172/2161-0991.S1-008
Copyright: © 2012 Jia C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: To introduce a method for harvesting pure hepatic vein (HV) blood and to determine time course and peak level of posthepatic (HV blood) reactive oxygen species (ROS) after liver transplantation. Materials and methods: Blood was taken from HV and infrahepatic vena cava (IH-VC) for malondialdehyde (MDA) detection at different time points after transplantation.
Results: Pure posthepatic blood from HV was successfully obtained using the technique of IH-VC cannulation. This method guaranteed the harvesting of pure posthepatic blood from HV and pure peripheral blood from IH-VC. MDA concentration in HV elevated obviously with time and peaked at 1h after transplantation. MDA concentration at 1h was significantly higher than that at other time points except for 5 min. Furthermore, MDA concentrations in HV were significantly higher than that in IH-VC at 20 min, 30 min, 1h ( P <0.05) and slightly higher at all other time points. MDA concentration within IH-VC peaked at 2h after transplantation.
Conclusion: We introduced a novel technique for harvesting pure posthepatic blood from HV using the manipulation of IH-VC cannulation. We provided the first evidence that posthepatic ROS concentration elevated with time and peaked at 1h after transplantation.