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ISSN: 2155-6180

Journal of Biometrics & Biostatistics
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Research Article

A Permutation Test of Genetic Association between Salmonella Isolated On-farm and At-abattoir using Amplified Fragment Length Polymorphism

Yingzhou Du1, Chong Wang1,2* and Peng Liu1

1Department of Statistics, Iowa State University, Ames, Iowa 50011, USA

2Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA

*Corresponding Author:
Chong Wang
Department of Statistics
Iowa State University, Ames
Iowa 50011, USA
Tel: 515-294-8047
Fax: 515-294-4040
E-mail: [email protected]

Received date: July 22, 2014; Accepted date: August 05, 2014; Published date: August 11, 2014

Citation: Du Y, Wang C, Liu P (2014) A Permutation Test of Genetic Association between Salmonella Isolated On-farm and At-abattoir using Amplified Fragment Length Polymorphism. J Biomet Biostat 5:204. doi:10.4172/2155-6180.1000204

Copyright: © 2014 Du Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are are credited.

Abstract

Pork and pork products have been identified as a significant source of Salmonella infection, which is a major public health concern. The contamination of Salmonella on pork can happen both on farms (before slaughter) and at abattoirs (after slaughter). Salmonella isolates were collected from both feces on farms and lymph nodes in the abattoir to determine if contamination at abattoirs can be linked back to the farms of origin. Molecular subtyping of the isolated Salmonella was performed using amplified fragment length polymorphism (AFLP), a Polymerase chain reaction-based, high-throughput, relatively inexpensive method. In this paper, we develop a permutation test for the genetic association of Salmonella isolated on-farm and at-abattoir using the AFLP data. Simulation studies show that the proposed permutation test controls the type I error rate appropriately as well as possesses high power. An application of the proposed permutation test to the real Salmonella ALFP data results in a p-value of 0.038 which shows strong evidence of association between Salmonella isolated on-farm and at-abattoir.

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