A Potential Biomarker for Acute Kidney Injury in Preterm Infants from Metabolic ProfilingLindsey E. Romick-Rosendale1, Kurt R. Schibler2, and Michael A. Kennedy1*
- *Corresponding Author:
- Michael A. Kennedy
Department of Chemistry and Biochemistry
Miami University, Oxford, Ohio 45056, USA
E-mail: [email protected]
Received date: December 09, 2011; Accepted date: January 28, 2012; Published date: February 02, 2012
Citation: Romick-Rosendale LE, Schibler KR, Kennedy MA (2012) A Potential Biomarker for Acute Kidney Injury in Preterm Infants from Metabolic Profiling. J Mol Biomark Diagn S3:001. doi:10.4172/2155-9929.S3-001
Copyright: © 2012 Romick-Rosendale LE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Metabolic profiles of hydrophilic and lipophilic cell extracts from three cancer cell lines, Miapaca-2, Panc-1 and AsPC-1, and a non-cancerous pancreatic ductal epithelial cell line, H6C7, were examined by proton nuclear magnetic resonance spectroscopy. Over twenty five hydrophilic metabolites were identified by principal component and statistical significance analyses as distinguishing the four cell types. Fifteen metabolites were identified with significantly altered concentrations in all cancer cells, e.g. absence of phosphatidylgrycerol and phosphatidylcholine, and increased phosphatidylethanolamine and cholesterols. Altered concentrations of metabolites involved in glycerophospholipid metabolism, lipopolysaccharide and fatty acid biosynthesis indicated differences in cellular membrane composition between non-cancerous and cancer cells. In addition to cancer specific metabolites, several metabolite changes were unique to each cancer cell line. Increased N-acetyl groups in AsPC-1, octanoic acids in Panc-1, and UDP species in Miapaca-2 indicated differences in cellular membrane composition between the cancer cell lines. Induced glutamine metabolism and protein synthesis in cancer cells were indicated by absence of glutamine other metabolites such as acetate, lactate, serine, branched amino acids, and succinate. Knowledge of the specifically altered metabolic pathways identified in these pancreatic cancer cell lines may be useful for identifying new therapeutic targets and studying the effects of potential new therapeutic drugs.