alexa A Quick Model for the Induction of Metabolic Syndrome M
ISSN: 2165-8048

Internal Medicine: Open Access
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Research Article

A Quick Model for the Induction of Metabolic Syndrome Markers in Rats

Barrios-Ramos JP1, Garduño-Siciliano L2*, Loredo-Mendoza ML4, Chamorro-Cevallos G2, Jaramillo-Flores ME1, Madrigal-Bujaidar E3 and Álvarez-González I3
1Graduates and Food Research, National School of Biological Sciences, National Polytechnic Institute, Prolongación de Carpio y Plan de Ayala s/n Colonia. Plutarco Elías Calles, Delegación Miguel Hidalgo, 11340 Mexico, D.F, Mexico
2Pharmacology Department, National School of Biological Sciences, National Polytechnic Institute, Prolongación de Carpio y Plan de Ayala s/n Colonia. Plutarco Elías Calles, Delegación Miguel Hidalgo, 11340 Mexico, D.F, Mexico
3Morphology Department, National School of Biological Sciences, National Polytechnic Institute, Prolongación de Carpio y Plan de Ayala s/n Colonia. Plutarco Elías Calles, Delegación Miguel Hidalgo, 11340 Mexico, D.F, Mexico
4School of Medicine, Panamerican University, Donatello 59 Mixcoac CP 03920, Mexico, D.F, Mexico
*Corresponding Author : Leticia Garduño-Siciliano
Escuela Nacional de Ciencias Biológicas
Instituto Politécnico Nacional, Ave. Wilfrido Massieu s/n
Esq. Manuel L. Stampa, Col. Unidad Profesional Adolfo López Mateos
Del. Gustavo A. Madero, C.P. 07738 Mexico, D.F., Mexico
Tel: +5255 5729 6300–52391
E-mail: [email protected]
Received October 16, 2013; Accepted January 28, 2014; Published February 05, 2014
Citation: Barrios-Ramos JP, Garduño-Siciliano L, Loredo-Mendoza ML, Chamorro- Cevallos G, Jaramillo-Flores ME, et al. (2014) A Quick Model for the Induction of Metabolic Syndrome Markers in Rats. Intern Med 4:137. doi:10.4172/2165-8048.1000137
Copyright: © 2014 Barrios-Ramos JP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

 Obesity, dyslipidemia, and glucose utilization disorders are the main indicators in the diagnosis of metabolic syndrome. However, there are other markers of damage from oxidative stress, such as the LDL cholesterol (LDL-c) plasma concentration, protein oxidation, lipoperoxidation, and DNA damage. In this study, male Wistar rats (250-270 g) were used. The rats were distributed into eight groups as follows: 1) standard diet and purified water intragastrically (1ml/kg) for seven weeks (N); 2, 3, 4, 5, 6 and 7) hypercholesterolemic diet and 60% fructose for four days (4D);one (1W),two (2W); three (3W), four (4W), five (5W), six (6W) and 7 (7W) weeks respectively and 8) 60% fructose for four weeks (4WF). At the end of treatment, the concentrations of total cholesterol (TC), HDL cholesterol (HDL-c), triglycerides (TGs), and glucose, as well as the alkaline phosphatase activity (ALP), were determined. Moreover, the percentage of cells with DNA migration was determined with a comet assay, and a histopathological study of the hepatic samples was conducted.

The animal model in the current study showed metabolic syndrome (MS) in animals after four weeks with the following well defined markers: decreased HDL-c; increased total cholesterol, LDL-c, atherogenic index, weight gain, and liver weight; hypertension; and defined steatosis leading to an increase in plasma ALP and genotoxic liver damage.

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