alexa A Recombinant Adenovirus Encoding Multiple HIV-1 Epitopes Induces Stronger CD4+ T cell Responses than a DNA Vaccine in Mice | OMICS International | Abstract
ISSN: 2157-7560

Journal of Vaccines & Vaccination
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Research Article

A Recombinant Adenovirus Encoding Multiple HIV-1 Epitopes Induces Stronger CD4+ T cell Responses than a DNA Vaccine in Mice

Daniela Santoro Rosa1,2,4, Susan Pereira Ribeiro1,2, Rafael Ribeiro Almeida1, Eliane Conti Mairena2,3, Jorge Kalil1,2,3 and Edecio Cunha- Neto1,2,3*

1Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, Brazil

2Institute for Investigation in Immunology-INCT, Sao Paulo, Brazil

3Heart Institute (InCor), University of Sao Paulo School of Medicine, Sao Paulo, Brazil

4Division of Immunology-Federal University of Sao Paulo-UNIFESP, Sao Paulo, Brazil

*Corresponding Author:
Edecio Cunha-Neto
Associate Professor
Division of Clinical Immunology and Allergy/LIM60
University of Sao Paulo School of Medicine (FM-USP)
Sao Paulo, SP Brazil, Avenida Dr. Arnaldo
455, sala 3209, Cerqueira Cesar
Sao Paulo, SP, Brazil, 01246-903
Tel: +55-11-30618315
Fax: +55-11-30618315
E-mail: [email protected]

Received date: September 01, 2011; Accepted date: November 25, 2011; Published date: December 02, 2011

Citation: Rosa DS, Ribeiro SP, Almeida RR, Mairena EC, Kalil J, et al. (2011) A Recombinant Adenovirus Encoding Multiple HIV-1 Epitopes Induces Stronger CD4+ T cell Responses than a DNA Vaccine in Mice. J Vaccines Vaccin 2:124. doi:10.4172/2157-7560.1000124

Copyright: © 2011 Saridi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

T-cell based vaccines against SIV/HIV may reduce both transmission and disease progression by inducing broad and functionally relevant T cell responses. Mounting evidence points toward a critical role for CD4+ T cells in the control of immunodeficiency and virus replication. We have previously shown that a DNA vaccine (HIVBr18), encoding 18 HIV CD4 epitopes capable of binding to multiple HLA class II molecules was able to elicit broad, polyfunctional, and long-lived CD4+ and CD8+ T cell responses in BALB/c and multiple HLA class II transgenic mice. By virtue of inducing broad responses against conserved CD4+ T cell epitopes that could be recognized across diverse common HLA class II alleles, this vaccine concept may cope with HIV-1 genetic variability and increase population coverage. Given the low immunogenicity of DNA vaccines in clinical trials, we tested the ability of a recombinant adenovirus serotype 5 encoding the 18 HIV epitopes (Ad5-HIVBr18) to increase specific cellular immune responses. We assessed the breadth and magnitude of HIV-specific proliferative and cytokine responses of CD4+ and CD8+ T cells induced by Ad5-HIVBr18 using different vaccination regimens/routes and compared to DNA immunization. Immunization with Ad5-HIVBr18 induced significantly higher specific CD4+ and CD8+ T cell proliferation, IFN-γ and TNF-α production than HIVBr18. The subcutaneous route of Ad5-HIVBr18 administration was associated with the highest responses. Ad5-HIVBr18 induced higher proliferative and cytokine responses than HIVBr18 up to 28 weeks post-immunization. Our results indicate that a vaccine based on an adenovirus vector encoding the HIVBr18 epitopes shows superior immunogenicity as compared to its DNA counterpart. These results support the possible testing of a vaccine encoding HIVBr18 in non-human primates and future clinical trials.

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