A Self-assembling Nanomaterial Reduces Acute Brain Injury and Enhances Functional Recovery in a Rat Model of Hypertensive Intracerebral HemorrhageLynn Yan-Hua Sang1,2,7, Yu-Xiang Liang3,7, Kwok-Fai So3,4,7,8,9, Gilberto Ka-Kit Leung1,7, Rutledge G Ellis-Behnke5,6,7
and Raymond Tak-Fai Cheung2,4*
- *Corresponding Author:
- Raymond Tak-Fai Cheung
Department of Medicine, University of Hong Kong
Administration Block, Queen Mary Hospital
Pokfulam, Hong Kong, China
E-mail: [email protected]
Received Date: July 25, 2014; Accepted Date: August 20, 2014; Published Date: August 27, 2014
Citation: Sang LY, Liang YX, So KF, Leung GK, Ellis-Behnke RG, et al. (2014) A Self-assembling Nanomaterial Reduces Acute Brain Injury and Enhances Functional Recovery in a Rat Model of Hypertensive Intracerebral Hemorrhage. J Nanomed Nanotechnol 5:224. doi: 10.4172/2157-7439.1000224
Copyright: © 2014 Sang LY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Intracerebral hemorrhage (ICH) carries a high morbidity and mortality rate. High systolic blood pressure promotes hematoma growth. A self-assembling peptide (SAP) can achieve immediate hemostasis via formation of a SAP nanofiber scaffold (SAPNS). A minimally invasive aspiration of hematoma plus local delivery of SAPNS may lead to decompression of brain tissue and prevent hematoma growth.
In a rat model of renovascular hypertension, experimental ICH was induced by a local injection of bacterial collagenase IV into the left basal ganglia. At 3.5 hours after induction of ICH, stereotactic clot aspiration or sham aspiration was performed manually. Following hematoma aspiration, an intrastriatal injection of 1% SAP, saline or sham injection was performed. Hematoma volume and brain swelling were quantified at 24 hours after ICH. Brain sections were immuno histochemically processed for myeloperoxidase and CD68 to detect the inflammatory infiltration in the perihematomal area. Perihematomal apoptotic cell death was determined using TUNEL staining. Functional recovery was assessed using neurological severity score and modified limb placement test at 1, 3, 7, 10 days after ICH.
The combined treatment with hematoma removal and locally delivered SAPNS decreased hematoma volume, hematoma growth, brain edema, perihematomal inflammatory cell infiltration and apoptosis, as well as improved sensorimotor functional recovery.
Locally delivered SAPNS after hematoma aspiration may prevent hematoma growth, facilitate the repair of ICHrelated brain injury and promote functional recovery. Such combined treatment may be effective in patients with hypertensive ICH.