A Sensitive and Interference-Free Liquid Chromatography Tandem Mass Spectrometry Method for Measuring Metanephrines in Plasma
- *Corresponding Author:
- Sihe Wang
Department of Clinical Pathology
Mail Code L11, 9500 Euclid Ave
Cleveland, OH 44195, USA
E-mail: [email protected]
Received Date: January 23, 2012; Accepted Date: February 14, 2012; Published Date: February 16, 2012
Citation: Gabler J, Yuan C, Woroniecki W, Liu H, Wang S (2012) A Sensitive and Interference-Free Liquid Chromatography Tandem Mass Spectrometry Method for Measuring Metanephrines in Plasma. J Chromatograph Separat Techniq 4: 195. doi: 10.4172/2157-7064.1000195
Copyright: © 2012 Gabler J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Plasma metanephrines are the primary biomarkers used to aid in diagnosing pheochromocytoma. However, the low physiological levels of metanephrines, physicochemical properties, and potential interferences make it challenging to achieve high sensitivity and specificity. In this report, we developed and validated a sensitive and interference-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring plasma metanephrines with simple sample preparation.
Results: Absolute ion suppression and matrix effect were observed, however, were completely compensated for by the internal standards. Epinephrine, an isobaric interferent of normetanephrine, was chromatographically separated, and no interference was observed from other common interferents. The method was linear from 0.08 to 22.2 nmol/L for normetanephrine and 0.03 to 8.2 nmol/L for metanephrine with accuracy ranging from 81 to 107%. No carryover was observed up to 56.8 nmol/L for normetanephrine and 8.7 nmol/L for metanephrine. Intra-assay and total CVs were within 6.8% for normetanephrine and 5.2% for metanephrine for three levels tested. Based on Deming regression, comparison with a reference LC-MS/MS method using patient specimens (n=40) showed a
slope of 0.973, intercept of 0.11 nmol/L and correlation coefficient of 0.9936 for normetanephrine and a slope of 1.039, intercept of -0.014 nmol/L, and correlation coefficient of 0.9914 for metanephrine. The mean difference was 3.5% and -1.6% for normetanephrine and metanephrine, respectively.
Conclusion: This LC-MS/MS assay is sensitive and free of interference for quantitation of plasma metanephrines