A Simple and Sensisitive HPLC Method to Monitor Serum and Synovial Fluid Concentrations of Ketorolac in Reumathologic Patients
Loretta Franceschi*, Sara D’aronco and Mario Furlanut
Chair of Pharmacology, Institute of Clinical Pharmacology and Toxicology, DPMSC, University of Udine, Udine, Italy
- *Corresponding Author:
- Dr. Loretta Franceschi
Chair of Pharmacology
Institute of Clinical Pharmacology and Toxicology
DPMSC,University of Udine, Piazzale SM
della Misericordia 15, 33100 Udine – Italy
Tel: +39 (0)432 559833
Fax: +39 (0)432 559819
E-mail: [email protected]
Received Date: August 26, 2010; Accepted Date: October 12, 2010; Published Date: October 12, 2010
Citation: Franceschi L, Furlanut M (2010) A Simple and Sensisitive HPLC Method to Monitor Serum and Synovial Fluid Concentrations of Ketorolac in Reumathologic Patients. J Bioanal Biomed 2:121-124. doi:10.4172/1948-593X.1000034
Copyright: © 2010 Franceschi L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A rapid, selective and sensitive isocratic reversed-phase HPLC assay coupled with UV detection for quanti fi cation of ketorolac in serum and synovial fl uid samples has been developed. Analytes were extracted on solid-phase cartridges (SPE) and chromatographic separation was achieved on a C18 column.
The chromatographic peak area ratio based on UV absorbency at 313 nm was used for quantitative analysis. This HPLC method has been successfully used for routine evaluation to monitor serum and synovial fl uid concentrations in reumathologic patients affering to our institute. Thanks to its sensitivity, this HPLC/UV method is also suitable for pharmacokinetic studies.