alexa A Simple and Sensitive Method for Quantitative Measurement of Methylmalonic Acid by Turbulent Flow Chromatography and Tandem Mass Spectrometry | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

A Simple and Sensitive Method for Quantitative Measurement of Methylmalonic Acid by Turbulent Flow Chromatography and Tandem Mass Spectrometry

Awet GT, Ryan CS, Lakshmi VR and Dean C Carlow*

Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA

*Corresponding Author:
Dean C Carlow
Department of Laboratory Medicine, Memorial
Sloan Kettering Cancer Center, New York, NY 10065, USA
Tel: 212- 639-8437
E-mail: [email protected]

Received date: September 08, 2016; Accepted date: September 15, 2016; Published date: September 21, 2016

Citation: Awet GT, Ryan CS, Lakshmi VR, Carlow DC (2016) A Simple and Sensitive Method for Quantitative Measurement of Methylmalonic Acid by Turbulent Flow Chromatography and Tandem Mass Spectrometry. J Chromatogr Sep Tech 7:336. doi:10.4172/2157-7064.1000336

Copyright: © 2016 Awet GT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple and sensitive method for the detection of methylmalonic acid in serum without derivatization has been developed. This method implements protein precipitation using methanol followed by additional sample clean up by turbulent flow liquid chromatography (TFLC). The sample was directly injected into the turbulent flow liquid chromatography tandem mass spectrometrysystem (TFLC-MS/MS) for online extraction followed by HPLC separation. The eluent was transferred to the mass spectrometer and ionized by heated electrospray negative ionization (HESI) and the analyte was quantified using a six-point calibration curve. The validated analytical measurement range (AMR) is 30-1,000 nMol/L. Dilutions of 10 and 200-fold were validated giving a clinical reportable range (CRR) of 30-200,000 nMol/L. The between-day and within-day imprecision values at concentrations spanning the AMR were less than 15%. This method was compared to an established LC-MS/MS method at a CLIA certified national reference laboratory and shows an excellent correlation with our TFLC-MS/

MS

method.

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