A Simple and Specific Method for Estimation of Lipoic Acid in Human Plasma by High Performance Liquid ChromatographyEzhilarasi K1, Sudha V2, Geetha Ramachandran2, Dhamodharan Umapathy1, Rama Rajaram3, Indira Padmalayam4, Vijay Viswanathan1 and Hemanth Kumar AK2*
1Department of Biochemistry and Molecular Genetics, Prof. M. Viswanathan Diabetes Research Centre and M.V. Hospital for Diabetes (WHO Collaborating Centre for Research, Education and Training in Diabetes), No. 4, West Madha Church Road, Royapuram, Chennai 600013, Tamil Nadu, India
- *Corresponding Author:
- Hemanth Kumar AK
Department of Biochemistry and Clinical Pharmacology
National Institute for Research in Tuberculosis (Indian Council of Medical Research)
Mayor Sathiyamoorthy Road, Chetpet, Chennai 600031, Tamil Nadu. India
E-mail: [email protected]
Received date: September 22, 2014; Accepted date: October 28, 2014; Published date: October 31, 2014
Citation: Ezhilarasi K, Sudha V, Ramachandran G, Umapathy D, Rajaram R, et al. (2014) A Simple and Specific Method for Estimation of Lipoic Acid in Human Plasma by High Performance Liquid Chromatography. J Chromatogr Sep Tech 5:245.. doi:10.4172/2157-7064.1000245
Copyright: 2014 Ezhilarasi K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A rapid high performance liquid chromatographic method for determination of lipoic acid in human plasma was developed and validated. The method involved extraction of lipoic acid in ethanol consisting of 50 mM disodium hydrogen phosphate: acetonitrile: methanol in the ratio of 50:30:20. The separation was done using a C18 column (150 mm) and detection was carried out using UV detection at 201 nm. The assay was found to be linear in the range of 0.78-50 μg/ml with the correlation coefficient of 0.9998. Intra and inter-day variations were determined by processing each standard concentration in duplicate for five consecutive days. The average recovery of lipoic acid from plasma was 113%. The developed method demonstrates better sensitivity, precision, accuracy, stability and linearity when compared with the methods previously used. The method is simple and can be used for the determination of lipoic acid in basic research studies as well as in standard clinical laboratories.