A Simple HPLC Assay for Ginsenoside-Rh2 in Plasma and Its Application for Pharmacokinetic Study in Rats
|Haijun Li1,2, Ping Ya Li2 and Pollen Yeung1*|
|1Pharmacokinetics and Metabolism Laboratory, College of Pharmacy and Department of Medicine, Dalhousie University, Halifax, NS, Canada|
|2Institute of Frontier Medical Science, Jilin University, Changchun, China|
|Corresponding Author :||Pollen KF Yeung, Ph.D.
College of Pharmacy, Dalhousie University
5968 College Street, Halifax
Nova Scotia, Canada
E-mail: [email protected]
|Received January 14, 2013; Accepted January 23, 2013; Published January 25, 2013|
|Citation: Li H, Li PY, Yeung P (2013) A Simple HPLC Assay for Ginsenoside-Rh2 in Plasma and Its Application for Pharmacokinetic Study in Rats. Nat Prod Chem Res 1:103. doi: 10.4172/2329-6836.1000103|
|Copyright: © 2013 Li H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Ginsenoside-Rh2 (G-Rh2) is an important constituent in ginseng and has significant anti-tumor property. A simple HPLC assay was developed to study pharmacokinetics of G-Rh2 in rat plasma. The HPLC consisted of a C18 reversed phase analytical column, a variable wavelength ultraviolet (UV) spectrophotometric detector, and a mobile phase made up of 0.005 M KH2PO4 (pH 7.2): acetonitrile: methanol (23:7:70). The system was operated at ambient temperature isocratically at a flow rate of 0.5 mL/min and wavelength at 203 nm. Extraction of G-Rh2 from plasma was achieved by solid phase extraction (SPE) using 100 mg/mL C18 SPE columns. The results showed that standard curves using 50 mL of plasma sample were linear from 0.25 to 100 mg/mL, with regression coefficient (r2)>0.99. The intra- and inter-assay variations over a 3-month study period were <10% and <20%, respectively. After 10 mg/kg twice daily for 4 doses by subcutaneous (sc) injection, the mean maximum plasma concentration (Cmax), and time to Cmax (Tmax) of G-Rh2 was 0.79 mg/mL and <0.5 hour, respectively. The described HPLC is readily performed in most laboratories and should have adequate sensitivity and specificity to study pharmacokinetics of G-Rh2 in rats following multiple doses.