A Simple HPLC Method for the Separation of Colistimethate Sodium and Colistin Sulphate
- *Corresponding Author:
- Gengliang Yang
Pharmaceutical Sciences College of Hebei University Baoding, Hebei-071002, China
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E-mail: [email protected]
Received date: December 27, 2010; Accepted date: February 09, 2011; Published date: February 12, 2011
Citation: Bai L, Ma Z, Yang G, Yang J, Cheng J (2011) A Simple HPLC Method for the Separation of Colistimethate Sodium and Colistin Sulphate. J Chromatograph Separat Techniq 1:105. doi:10.4172/2157-7064.1000105
Copyright: © 2011 Bai L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this paper, a simple high performance liquid chromatography (HPLC) method for the determination of colistimethate sodium in the productions of synthesis from colistin E sulphate was established. A HPLC gradient which was: 20% B+80% A changed to 50% B+50% A in 10 min (A was 0.05% TFA aqueous solution and B was acetonitrile) was used and the separation was realized in 8 minutes. Moreover, this method was also used in the separation of colistin sulphate with good resolution. Compared to the methods reported previously, the present method was finished easier in a much shorter time which is 6 min. LC-MS was used to detect colistin sulphate and the result showed that the two compositions were colistin sulphate E1 and E2 as expected. Good separation and reproducibility were obtained.