A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
Natacha E Piloni and Susana Puntarulo*
Institute of Biochemistry and Molecular Medicine, University of Buenos Aires, Conicet, Physical Chemistry, Faculty of Pharmacy and Biochemistry, Junín, Buenos Aires, Argentina
- Corresponding Author:
- Puntarulo S
Fisicoquímica, Institute of Biochemistry and Molecular Medicine
Faculty of Pharmacy and Biochemistry Junín 956
CAAD1113, Buenos Aires, Argentina
E-mail: [email protected]
Received Date: July 13, 2016; Accepted Date: November 10, 2016; Published Date: November 14, 2016
Citation: Piloni EN, Puntarulo S (2016) A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload. Bioenergetics 5:e125. doi:10.4172/2167-7662.1000e125
Copyright: © 2016 Piloni NE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
All biological systems, contain many antioxidants; including water-soluble compounds, such as ascorbic acid. The one-electron oxidation of ascorbate (AH¯produces the ascorbyl radical (A•), that has a relatively long lifetime compared to other species, such as hydroxyl radical, peroxyl, alkoxyl, and carbon-centered lipid free radicals. A• has been proposed as a marker of oxidative stress either in vitro, or in vivo in numerous systems. The application of a simple kinetic analysis allowed us to estimate the steady state concentration of A•. These data were successfully compared to experimental values obtained using Electron Paramagnetic Resonance (EPR) in rat brain under physiological conditions. The model was also applied to estimate the response to stress due to subchronic Fe overload.