A Simple Method of Generating Complex DNA Profiles Utilizing Alu-based Markers with Applications in Forensics, Paternity, Genetic Mapping, Population Studies and Ancestry
David H Kass*
Department of Biology, 441 Mark Jefferson Ypsilanti, MI 48197 USA
- *Corresponding Author:
- David H Kass
Department of Biology
441 Mark Jefferson Ypsilanti
MI 48197 USA
E-mail: [email protected]
Received date: July 17, 2013; Accepted date: September 23, 2013; Published date: September 27, 2013
Citation: Kass DH (2013) A Simple Method of Generating Complex DNA Profiles Utilizing Alu-based Markers with Applications in Forensics, Paternity, Genetic Mapping, Population Studies and Ancestry. J Forensic Res 4:200. doi:10.4172/2157-7145.1000200
Copyright: © 2013 Kass DH. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
As DNA profiling systems become more complex, advancements to a relatively simple technique are presented that promote greater accessibility and usefulness for a variety of applications. In contrast, other simple tools commonly used to teach students about forensics and human populations have notable drawbacks. Two Alutetraplex systems, utilizing four Alu presence/absence variants in a single reaction were therefore developed to provide a simple methodology to generate complex profiles. A third Alutetraplex system is presented, escalating the number of possible genotypes to 531,441, with all alleles of the 12 dimorphic markers being relatively common. Reproducible results were attained even with the use of crude DNA preparations stored frozen for several years with multiple freeze thaws. The incorporation of GelRed DNA stain instead of the highly toxic ethidium bromide promotes greater accessibility, particularly in a classroom setting. This study demonstrates the effectiveness of this profiling system as a simple but informative methodology to analyze paternity, genetic mapping of human traits, and provides data to illustrate its potential in assessing ancestry or geographic origins of an individual.