alexa A Simple Method to Quantify Glycogen from Human Platelets
ISSN: 2157-7099

Journal of Cytology & Histology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

A Simple Method to Quantify Glycogen from Human Platelets

Débora Santos Rocha1, Samir Khal de Souza1, Tor Gunnar Hugo Onsten2, Roselis Silveira Martins da Silva3 and Marcos Emílio Frizzo1,2*

1Department of Morphological Sciences, Universidade Federal do Rio Grande do Sul, Brazil

2Hemotherapy Service, Clinical Hospital of Porto Alegre, Universidade Federal do Rio Grande do Sul, Brazil

3Department of Physiology, Universidade Federal do Rio Grande do Sul, Brazil

*Corresponding Author:
Marcos Emílio Frizzo
Department of Morphological Sciences and Hemotherapy Service
Clinical Hospital of Porto Alegre
Universidade Federal do Rio Grande do Sul, Brazil
Tel: 55 51 33084536
Fax: 55 51 33083146
E-mail: [email protected]

Received Date: December 19, 2013; Accepted Date: February 04, 2014; Published Date: February 06, 2014

Citation: Rocha DS, de Souza SK, Onsten TGH, da Silva RSM, Frizzo ME (2014) A Simple Method to Quantify Glycogen from Human Platelets. J Cytol Histol 5:217. doi: 10.4172/2157-7099.1000217

Copyright: © 2014 Rocha DS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: The aim of our study was to establish a protocol to quantify the glycogen from human platelets. Methods: The total glycogen was determined in the platelet concentrate (PC) obtained from healthy male volunteers. The assay comprised the following steps: (1) cell lysing and glycogen extraction, (2) precipitation of glycogen and its washing, (3) conversion of glycogen to free glucose, (4) neutralization, and (5) detection of free glucose. Results: In our analysis, the measured glycogen content in the PC was 0.263 ± 0.101 μg/106 platelets (mean ± SD, n=16). Discussion: Despite the potential importance of glycogen determination in the platelets, a biochemical method to quantify this polymer in these cells is not available at the moment. Our protocol proved to be a simple method to measure these reserves from a blood sample. We expect to contribute to future studies of platelet metabolism, especially regarding glycogen function in these cells.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords