A Simple Method to Quantify Glycogen from Human Platelets
- *Corresponding Author:
- Marcos Emílio Frizzo
Department of Morphological Sciences and Hemotherapy Service
Clinical Hospital of Porto Alegre
Universidade Federal do Rio Grande do Sul, Brazil
Tel: 55 51 33084536
Fax: 55 51 33083146
E-mail: [email protected]
Received Date: December 19, 2013; Accepted Date: February 04, 2014; Published Date: February 06, 2014
Citation: Rocha DS, de Souza SK, Onsten TGH, da Silva RSM, Frizzo ME (2014) A Simple Method to Quantify Glycogen from Human Platelets. J Cytol Histol 5:217. doi: 10.4172/2157-7099.1000217
Copyright: © 2014 Rocha DS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: The aim of our study was to establish a protocol to quantify the glycogen from human platelets. Methods: The total glycogen was determined in the platelet concentrate (PC) obtained from healthy male volunteers. The assay comprised the following steps: (1) cell lysing and glycogen extraction, (2) precipitation of glycogen and its washing, (3) conversion of glycogen to free glucose, (4) neutralization, and (5) detection of free glucose. Results: In our analysis, the measured glycogen content in the PC was 0.263 ± 0.101 μg/106 platelets (mean ± SD, n=16). Discussion: Despite the potential importance of glycogen determination in the platelets, a biochemical method to quantify this polymer in these cells is not available at the moment. Our protocol proved to be a simple method to measure these reserves from a blood sample. We expect to contribute to future studies of platelet metabolism, especially regarding glycogen function in these cells.