alexa A Simplified Method for Measuring Secreted Invertase Ac
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
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Research Article

A Simplified Method for Measuring Secreted Invertase Activity in Saccharomyces cerevisiae

Harkness Troy AA* and Arnason Terra G#
Departments of Anatomy and Cell Biology
#College of Medicine, University of Saskatchewan, Saskatoon, Canada
Corresponding Author : Harkness Troy AA
Department of Anatomy and Cell Biology
University of Saskatchewan, B313 Health Sciences Building
107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada
Tel: (306) 966-1995
Fax: (306) 966-4298
E-mail: [email protected]
Received September 09, 2014; Accepted October 28, 2014; Published November 04, 2014
Citation: Harkness Troy AA, Arnason Terra G (2014) A Simplified Method for Measuring Secreted Invertase Activity in Saccharomyces cerevisiae. Biochem Pharmacol (Los Angel) 3:151. doi:10.4172/2167-0501.1000151
Copyright: © 2014 Harkness Troy AA .This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The measurement of sucrose hydrolysis to glucose and fructose in yeast is valuable for many aspects of yeast cell biology and metabolic analysis, and has been used extensively to understand the genetics of the SNF1 kinase. However, a simple and rapid method describing how to perform this assay for new users is lacking. Here, we review various methods used to measure invertase activity. We focus on a method based on a colorimetric assay that was first described by Goldstein and Lampen in 1975. Our report not only describes a simple and rapid application of this method to measure secreted invertase enzymatic activity from yeast whole cells for new users, but also establishes optimized times for assay conditions. The method utilizes a small volume of yeast culture in which the entire procedure can be performed in less than an hour. Very few reports offer a step-by-step method that can be readily used without prior knowledge of the procedure, providing the impetus for this report. The optimizations described in this report allow increased sensitivity when comparing subtle changes.


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