alexa A Simplified Method for Measuring Secreted Invertase Ac
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

A Simplified Method for Measuring Secreted Invertase Activity in Saccharomyces cerevisiae

Harkness Troy AA* and Arnason Terra G#
Departments of Anatomy and Cell Biology
#College of Medicine, University of Saskatchewan, Saskatoon, Canada
Corresponding Author : Harkness Troy AA
Department of Anatomy and Cell Biology
University of Saskatchewan, B313 Health Sciences Building
107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada
Tel: (306) 966-1995
Fax: (306) 966-4298
E-mail: [email protected]
Received September 09, 2014; Accepted October 28, 2014; Published November 04, 2014
Citation: Harkness Troy AA, Arnason Terra G (2014) A Simplified Method for Measuring Secreted Invertase Activity in Saccharomyces cerevisiae. Biochem Pharmacol (Los Angel) 3:151. doi:10.4172/2167-0501.1000151
Copyright: © 2014 Harkness Troy AA .This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The measurement of sucrose hydrolysis to glucose and fructose in yeast is valuable for many aspects of yeast cell biology and metabolic analysis, and has been used extensively to understand the genetics of the SNF1 kinase. However, a simple and rapid method describing how to perform this assay for new users is lacking. Here, we review various methods used to measure invertase activity. We focus on a method based on a colorimetric assay that was first described by Goldstein and Lampen in 1975. Our report not only describes a simple and rapid application of this method to measure secreted invertase enzymatic activity from yeast whole cells for new users, but also establishes optimized times for assay conditions. The method utilizes a small volume of yeast culture in which the entire procedure can be performed in less than an hour. Very few reports offer a step-by-step method that can be readily used without prior knowledge of the procedure, providing the impetus for this report. The optimizations described in this report allow increased sensitivity when comparing subtle changes.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords