A Sub-Chronic Exposure Study of Arsenic on Hematological Parameters, Liver Enzyme Activities, Histological Studies and Accumulation Pattern of Arsenic in Organs of Wistar Albino RatsAl-Forkan M1*, Islam S2, Akter R3, Shameen Alam S1, Khaleda L1, Rahman Z2 and Salma Chowdhury DU1
- *Corresponding Author:
- Al-Forkan Md
Department of Genetic Engineering and Biotechnology
University of Chittagong, Chittagong- 4331, Bangladesh
Fax: + 88-031-2606014
E-mail: [email protected]
Received Date: Nov 23, 2015; Accepted Date: Feb 15, 2016; Published Date: Feb 26, 2016
Citation: Al-Forkan M, Islam S, Akter R, Shameen Alam S, Khaleda L, et al. (2016) A Sub-Chronic Exposure Study of Arsenic on Hematological Parameters, Liver Enzyme Activities, Histological Studies and Accumulation Pattern of Arsenic in Organs of Wistar Albino Rats. J Cytol Histol S5: 06. doi:10.4172/2157-7099.S5-006
Copyright: © 2016 Al-Forkan Md, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The aim of this study was to determine the hematological and biochemical changes occurred in the blood of arsenic-exposed Wistar albino rats during 90 days sodium-arsenite exposure study, and additionally to investigate the histological injury in the organs (liver, kidney, spleen and heart) of Wistar rats along with identifying the accumulation pattern of arsenic in those organs. Wistar rats received the following treatments: (1) distilled water; (2) arsenic (10 ppm); (3) arsenic (30 ppm) (4) arsenic (50 ppm). We found anemia, immunosuppression as well as significant increase in liver enzyme activity in the blood of arsenic intoxicated rats. Moreover, this toxic metalloid produced marked necrosis in liver and kidney of rats, whereas in heart mild muscle necrosis was observed. In case of spleen, enlarged white pulp was seen with marked hemorrhage and necrosis. Besides, we also have found that liver and spleen accrued more arsenic than kidney and heart. So, for better understanding of the toxic effects of arsenic the findings of our study in animal model might become useful which will also aid to develop effective drug against arsenic mediated toxic effects on human health.