alexa A Versatile Metagenome Purification Method to Identify Uncultivable Bacteria by Denaturing Gradient Gel Electrophoresis (DGGE) from Sediments and Soils | Abstract
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

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A Versatile Metagenome Purification Method to Identify Uncultivable Bacteria by Denaturing Gradient Gel Electrophoresis (DGGE) from Sediments and Soils

Lourdes Reyes-Escogido1, Ingrid Rodríguez-Buenfil1, Jesús Valdés2, Luis Kameyama3, Odila Saucedo Cárdenas4 and Francisco Martínez- Pérez1*

1Unidad Sureste, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., México

2Departamento de Bioquímica, Centro del Investigación y de Estudios Avanzados del I.P.N., México

3Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., México

4Departamento de Histología, Facultad de Medicina, Universidad Autónoma de Nuevo Léon and División de Genética, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, N.L., México

*Corresponding Author:
Francisco Martínez-Pérez
Unidad Sureste
Centro de Investigación y Asistencia en
Tecnología y Diseño del Estado de Jalisco
A.C, Colonia Garcia Gineres
Merida 97070, Yucatan, México
Tel: +52 9999 202671
Fax: +52 9999 202671
E-mail: [email protected]

Received date: August 06, 2012; Accepted date: August 29, 2012; Published date: August 30, 2012

Citation: Reyes-Escogido L, Rodríguez-Buenfil I, Valdés J, Kameyama L, Cárdenas OS, et al. (2012) A Versatile Metagenome Purification Method to Identify Uncultivable Bacteria by Denaturing Gradient Gel Electrophoresis (DGGE) from Sediments and Soils. J Bacteriol Parasitol 3:147. doi: 10.4172/2155-9597.1000147

Copyright: © 2012 Reyes-Escogido L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Here, we report a versatile metagenome purification method to identify uncultivable species from sediments and soils by nested-PCR-DGGE according to 16S rDNA. This combination of methods uses enzymatic lysis in situ, polyvinylpyrrolidone (PVP), Chelex 100, glass bead-silica gel and chaotropic salts, with the advantage that it can be applied to different soils.

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