Review Article
Accuracy of Next Generation Sequencing Platforms
Edward J Fox1, Kate S Reid-Bayliss1, Mary J Emond2 and Lawrence A Loeb1*1Departments of Pathology and Biochemistry, University of Washington, USA
2Department of Biostatistics, University of Washington, USA
- *Corresponding Author:
- Lawrence A Loeb
Departments of Pathology and Biochemistry
University of Washington, USA
Tel: 1-206-543-0556
Fax: 1-206-543-3967
E-mail: [email protected]
Received date: April 30, 2014; Accepted date: June 26, 2014; Published date: June 28, 2014
Citation: Fox EJ, Reid-Bayliss KS, Emond MJ, Loeb LA (2014) Accuracy of Next Generation Sequencing Platforms. Next Generat Sequenc & Applic 1:106. doi:10.4172/2469-9853.1000106
Copyright: © 2014 Fox EJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Next-generation DNA sequencing has revolutionized genomic studies and is driving the implementation of precision diagnostics. The ability of these technologies to disentangle sequence heterogeneity, however, is limited by their relatively high error rates. A Several single molecule barcoding strategies have been propose to reduce the overall error frequency. A Duplex Sequencing additionally exploits the fact that DNA is double-strand, with one strand reciprocally encoding the sequence information of its complement, and can eliminate nearly all sequencing errors by comparing the sequence of individually tagged amplicons derived from one strand of DNA with that of its complementary strand. This method reduces errors to fewer than one per ten million nucleotides sequenced.
