alexa Achroma Software-High-Quality Policy in (a-)Typical Mass Spectrometric Data Handling and Applied Functional Proteomics | OMICS International
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
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Research Article

Achroma Software-High-Quality Policy in (a-)Typical Mass Spectrometric Data Handling and Applied Functional Proteomics

Michael Krappmann1#, Christine M Kaufmann2#, Romy K Scheerle2, Johanna Grassmann2 and Thomas Letzel2*

1University of Applied Sciences, Am Hofgarten 4, 85354 Freising, Weihenstephan, Germany

2Chair of Urban Water Systems Engineering, TU München, Am Coulombwall, 85748 Garching, Germany

#Equal contribution of the authors

*Corresponding Author:
Thomas Letzel
Chair of Urban Water Systems Engineering
TU München, Am Coulombwall
85748 Garching, Germany
Tel: +49 (0)89 2891 3780
Fax: +49 (0)89-289-13718
E-mail: [email protected]

Received Date: July 03, 2014; Accepted Date: August 25, 2014; Published Date: August 28, 2014

Citation: Krappmann M, Kaufmann CM, Scheerle RK, Grassmann J, Letzel T (2014) Achroma Software-High-Quality Policy in (a-)Typical Mass Spectrometric Data Handling and Applied Functional Proteomics. J Proteomics Bioinform 7: 264-271. doi: 10.4172/jpb.1000328

Copyright: © 2014 Krappmann M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Data evaluation of mass spectrometric raw data is an essential step to obtain high-quality results. In our days an exorbitant amount of raw data are produced in analytical (bio)chemistry due to the utilization of sophisticated experimental setups. The recently published free software Achroma has been developed to overcome increasing data processing challenges by providing the possibility of a comprehensive data analysis (https://openmasp.hswt. de/pages/project/achroma.php). To illustrate (a-)typical data evaluation, an online coupled continuous flow system hyphenated with mass spectrometric detection was applied to investigate enzymatic activity changes in the presence of regulatory molecules and alternative substrates. The extended software strategy, the processing as well as evaluation of data is presented in detail based on enzymatic assays of intestinal alkaline phosphatase (iAP) and acetylcholine esterase (AChE). Different Achroma data evaluation modules enabled a high quality analysis. This included the elucidation of enzymatic substrate preferences by means of the calculation of negative and positive peak areas as well as the identification of an inhibitory molecule by comparing different mass spectra with regard to their overall composition. The possibility of an automatically performed validity control to monitor the systems robustness furthermore emphasized the usefulness of Achroma software regarding its applicability in the area of 'functional proteomics' data handling.

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