Acute Leukemia Trilineage Blasts Assignment and Epigenetic MutationsAmal M*, Ines S, Mbarka B, Chaker F and Salem A
Laboratory of Molecular and Cellular Hematology, Medicine Tunis Faculty, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia
- Corresponding Author:
- Mechaal A
Laboratory of Molecular and Cellular Hematology
Pasteur Institute of Tunis, University of Tunis El Manar
Tunis, 13 Place Pasteur, BP 74, 1002 Tunis Belvedere Tunisia
Tel: +216 71-873-366
Fax: +216 71-872-055
E-mail: [email protected]
Received Date: April 02, 2017; Accepted Date: April 16, 2017; Published Date: April 18, 2017
Citation: Amal M, Ines S, Mbarka B, Chaker F, Salem A (2017) Acute Leukemia Trilineage Blasts Assignment and Epigenetic Mutations. J Bone Res 5:174. doi:10.4172/2572-4916.1000174
Copyright: © 2017 Mechaal A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mixed Phenotype Acute Leukaemia (MPAL) is a heterogeneous group of rare leukemias in which assigning a single lineage of origin is not possible.
We report a rare case of a MPAL without any clear cytological characterisation. Immuno-phenotyping identifies a blastic population co-expressing myeloid, B-lymphoid and T-lymphoid markers. The diagnosis of MPAL is considered and the patient receives chemotherapy targeting both myeloid and lymphoid components, followed by allogeneic hematopoietic stem cell transplantation. DNA-based techniques analyzing B and T-cell clonality identified the presence of partial rearrangements in immunoglobulin and TCR genes. Mutations in DNMT3A, IDH1/2, Flt3, NPM1 and ASXL1 genes were analysed.
We did not observe any mutations in IDH2, Flt3 and ASXL1 genes. A several mutations were in DNMT3A, IDH1 and NPM1 genes, allowing the monitoring of minimal residual disease.