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Adaptation of the Micronucleus Technique in <em>Allium Cepa</em>, For Mutagenicity Analysis of the Jamari River Valley, Western Amazon, Brazil | OMICS International | Abstract
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
Open Access

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Research Article

Adaptation of the Micronucleus Technique in Allium Cepa, For Mutagenicity Analysis of the Jamari River Valley, Western Amazon, Brazil

Dionatas Ulises de Oliveira Meneguetti1*, Francisco Carlos da Silva2, Renato André Zan3 and
Leandro José Ramos4
1Department of biology, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil
2Department of biology, Centro Universitário Luterano de Ji-paraná, Rondônia, Brasil
3Department of chemistry, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil
4Department of Health, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil
Corresponding Author : Dionatas Ulises de Oliveira Meneguetti
Department of biology /FAEMA. Av. Machadinho 4349
Setor 06, 76873-630 Ariquemes, RO, Brazil
Tel: 55 69 3536-6600
E-mail: [email protected]
Received January 19, 2012; Accepted February 27, 2012; Published February 29, 2012
Citation: de Oliveira Meneguetti DU, da Silva FC, Zan RA, Ramos LJ (2012) Adaptation of the Micronucleus Technique in Allium Cepa, For Mutagenicity Analysis of the Jamari River Valley, Western Amazon, Brazil. J Environment Analytic Toxicol 2:127. doi: 10.4172/2161-0525.1000127
Copyright: © 2012 de Oliveira Meneguetti DU, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

This study aimed to make technique adjustment of micronuclei in Allium cepa, recommended by Fiskejö [1], by replacing methanol and Giemsa by triarilmetano the 0,1%, xantenos the 0,1% and tiazinas the 0,1%. We obtained good results with the replacement, as its efficiency in stain nucleic acids, obtaining a good visualization of cells, mitotic index, micronuclei, anaphase and telophase bridges, and this methodology is indicated in future examinations of mutagenicity in the river Vale do Jamari.

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