Adhesion to Extracellular Matrix Proteins can Differentiate Between Human Bone Marrow Derived Mesenchymal Stem Cells and FibroblastsLorenzo P Roncoroni1#, Jan K Maerz1#, Brigitte Angres2,3, Heiko Steuer2, Karin Benz2, Tanja Abruzzese1, Melanie L Hart1, Bernd Rolauffs4, Gerd Klein5, Dieter Stoll6 and Wilhelm K Aicher1*
- Corresponding Author:
- Wilhelm K Aicher, PhD
Department of Urology
University of Tuebingen Hospital
72076 Tuebingen, Germany
Tel: +49 7071 298 7020
Fax: +49 7071 292 5072
E-mail: [email protected]
Received date: February 26, 2013; Accepted date: March 26, 2013; Published date: March 28, 2013
Citation: Roncoroni LP, Maerz JK, Angres B, Steuer H, Benz K, et al. (2013) Adhesion to Extracellular Matrix Proteins can Differentiate between Human Bone Marrow Derived Mesenchymal Stem Cells and Fibroblasts. J Tissue Sci Eng S11:008. doi:10.4172/2157-7552.S11-008
Copyright: © 2013 Roncoroni LP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mesenchymal stem or stromal cells (MSC) contribute in vivo to wound repair and can be utilized for tissue regeneration. In contrast, fibroblasts may contribute to scar formation and may even hamper functional regeneration. Depending on the clinical application, MSC are sometimes attached to a scaffold to maintain the cells in the area of regeneration. We therefore screened for proteins that allow a preferential binding of MSC and avoid strong adherence of fibroblasts. The human MSC were isolated from bone marrow (bmMSC) or term placenta (pMSC). Synovial fibroblasts (SF) and dermal fibroblasts (DF) served as controls. In the first set of experiments, binding of bmMSC and SF to extracellular matrix (ECM) proteins was investigated by multiple substrate array (MSA®). From MSA® protein analyses 57 peptides with potential MSC-binding sites were selected and the binding of the cells to these peptides was determined. We report that MSC differ from fibroblasts in their binding to proteins of the extracellular matrix. MSC bind with higher efficiency to laminin-111, collagens-I, -III, and -IV and tenascin-C compared to fibroblasts, while both cell types bind with high efficiency to fibronectin, vitronectin, and laminin-511. We conclude that overall MSC seem less selective with respect to binding extracellular matrix components compared to fibroblasts, and fibroblasts attach to fewer proteins and peptides.