alexa Advancements in Characterization Techniques of Biopolym
ISSN: 2379-1764

Advanced Techniques in Biology & Medicine
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Review Article

Advancements in Characterization Techniques of Biopolymers: Cyclic Voltammetry, Gel Electrophoresis, Circular Dichroism and Fluorescence Spectroscopy

Mahima Kaushik1,2*, Mohan Kumar2, Swati Chaudhary2, Swati Mahendru2 and Shrikant Kukreti2*

1Cluster Innovation Centre, University of Delhi, Delhi, India

2Nucleic Acids Research Laboratory, Department of Chemistry, University of Delhi, Delhi, India

*Corresponding Authors:
Mahima Kaushik
Associate Professor
Cluster Innovation Centre
University of Delhi, Delhi, India
Tel: 911127666702
E-mail: [email protected]; [email protected]
 
Shrikant Kukreti
Professor, Nucleic Acids Research Laboratory
Department of Chemistry
University of Delhi, Delhi, India
Tel: 911127666726
E-mail: [email protected]; [email protected]

Received Date: June 25, 2016; Accepted Date: July 02, 2016; Published Date: July 09, 2016

Citation: Kaushik M, Kumar M, Chaudhary S, Mahendru S, Kukreti S (2016) Advancements in Characterization Techniques of Biopolymers: Cyclic Voltammetry, Gel Electrophoresis, Circular Dichroism and Fluorescence Spectroscopy. Adv Tech Biol Med 4:184. doi: 10.4172/2379-1764.1000184

Copyright: © 2016 Kaushik M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Biopolymers such as DNA, RNA and proteins are the key players in the cellular processes like cell differentiation, cell growth, maintenance, repair, recombination, transcription, translation, etc. Immense efforts have been made for their isolation, purification, quantification and structural as well as functional characterization. Profound insights into the structural and functional characterization of these biopolymers could help us understand the intricate cellular machinery. A wide range of biochemical, biophysical, electrochemical and molecular biology techniques have really been beneficial in exploring the key and interdependent relationships between the structure and function of these biopolymers. Each instrumentation technique has its own advantage and disadvantage in terms of their applications, selectivity and sensitivity. There had been a lot of advancements in these techniques for achieving their desired goals, but still, lots of limitations need our attention and further improvements. For understanding, the extremely complicated biological machinery at molecular and cellular level, every small fragment of a cell has to be studied separately with a lot of intricacies. In this review, we present a brief update of the advancements made in the large repertoire of Cyclic Voltammetry (CV), Gel electrophoresis, Circular Dichroism (CD) and Fluorescence spectroscopy techniques which might be relevant for updating our knowledge of the pre-existing and well-established tools utilized by the scientists across the world.

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