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ISSN: 2168-9547

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Review Article

Advances in Molecular Techniques Used Flax Research in China

Guangwen Wu1*,Ying Yu1, Hongmei Yuan1, Jianzhong Wu1, Yan Liu1, Si Chen1, Lili Cheng1, Qinghua Kang1,Wengong Huang1, Dongwei Xie1, Yubo Yao1, Xixia.Song1, Liguo Zhang1, Fengzhi Guang1* and Krzysztof Heller2

1Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China

2Institute of Natural Fibers and Medicinal Plants, Poznan, Poland

*Corresponding Author:
Fengzhi Guang
Institute of Industrial Crops
Heilong jiang Academy of Agricultural Sciences
Harbin 150086, PR China
Tel: +86 451 8666 2295
E-mail: [email protected]
 
Guangwen Wu
Institute of Industrial Crops
Heilong jiang Academy of Agricultural Sciences
Harbin 150086, PR China
E-mail: [email protected]

Received date: October 26, 2015; Accepted date: November 03, 2015; Published date: November 10, 2015

Citation: Wu G, Yu Y, Yuan H, Wu J, Liu Y, et al. (2015) Advances in Molecular Techniques Used Flax Research in China. Mol Biol 4:146. doi: 10.4172/2168-9547.1000146

Copyright: © 2015 Wu G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In this paper, the major achievements in the application of molecular methods in flax research in China are discussed. Some advanced biological technologies in flax breeding program are mentioned that were utilized widely in flax studies, which were also in employed in the fiber crop sector around the world. Special attention has been focused on the new results of flax disease investigations performed through molecular methods, particularly those related to powdery mildew and wilt. A conclusion was drawn that powdery mildew resistance is inherited via a single dominant gene. A label bank was made containing 20000 SAGE LABLE separating from cDNA, through SAGE analysis of genes resistant to powdery mildew. AFLP analysis was performed on wilt. Specific bands, AG/CAG and FuJ7(t), of the wilt resistance gene were found to be closely linked, with a genetic distance of 5.2 cm between them. The AG/CAG segments were recovered, cloned, sequenced, and successfully transformed into a SCAR marker, used for molecular detection and marker-assisted selection breeding. A flax genetic linkage map was constructed with 12 linkage groups. The results revealed that the markers on the map were distributed evenly, and the co-dominant markers in SRAP and SSR were more suitable for the construction of a genetic map in flax. All of the above findings have established a solid foundation for further flax research in China.

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