alexa AFM/TEM Complementary Structural Analysis of Surface-Functionalized Nanoparticles | OMICS International| Abstract

ISSN: 2161-0398

Journal of Physical Chemistry & Biophysics

  • Review Article   
  • J Phys Chem Biophys 2014, Vol 4(4): 150
  • DOI: 10.4172/2161-0398.1000150

AFM/TEM Complementary Structural Analysis of Surface-Functionalized Nanoparticles

Ruozi B1*, Belletti D1, Vandelli MA1, F Pederzoli1, P Veratti1, F Forni1, G Tosi1, Tonelli M2 and Zapparoli M2
1Department of Life Sciences, University of Modena and Reggio Emilia, Italy
2CIGS, Centro Interdipartimentale Grandi Strumenti, University of Modena and Reggio Emilia, Italy
*Corresponding Author : Ruozi B, Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41124, Modena, Italy, Tel: +39.059.2055128, Fax: +39.0592055131, Email: [email protected]

Received Date: May 08, 2014 / Accepted Date: Jun 28, 2014 / Published Date: Jun 30, 2014

Abstract

In the field of nanomedicine, the characterization of functionalized drug delivery systems, introduced on market as efficacious and selective therapeutics, represents a pivotal aspect of great importance. In particular, the morphology of polymeric nanoparticles, the most studied nanocarriers, is frequently assessed by transmission electron microscopy (TEM). Despite of TEM high resolution and versatility, this technology is frequently hampered by both the complicated procedure for sample preparation and the operative condition of analysis. Considering the scanning probe microscopies, atomic force microscopy (AFM) represents an extraordinary tool for the detailed characterization of submicron-size structure as the surface functionalization at the atomic scale. In this paper we discussed the advantage and limits of these microscopies applied to the characterization of PLGA nanoparticles functionalized with three different kinds of ligands (carbohydrate ligand, an antibody and quantum dots crystals) intentionally designed, created and tailored with specific physico-chemical properties to meet the needs of specific applications (targeting or imaging).

Keywords: Transmission electron microscopy (TEM); Atomic force microscopy (AFM); PLGA; Nanoparticles; Surface functionalization; Neu5Ac; Rituximab; Quantum Dots (QD)

Citation: Ruozi B, Belletti D, Vandelli MA, Pederzoli F, Veratti P, et al. (2014) AFM/ TEM Complementary Structural Analysis of Surface-Functionalized Nanoparticles. J Phys Chem Biophys 4:150. Doi: 10.4172/2161-0398.1000150

Copyright: © 2014 Ruozi B. et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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