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Alcaligenes-The 4T Engine Oil Degrader
| Hardik Pathak* and Kamna Bhatnagar | |
| Department of Biotechnology, Mahatma Gandhi Institute of Applied Sciences, Jaipur, India | |
| Corresponding Author : | Dr. Hardik Pathak Head Department of Biotechnology Mahatma Gandhi Institute of applied sciences, JECRC Campus Tonk Road, Jaipur, India Tel: +919602453825 E-mail: hardikaeshu@gmail.com |
| Received June 24, 2011; Accepted September 12, 2011; Published September 16, 2011 | |
| Citation:Pathak H, Bhatnagar K (2011) Alcaligenes-The 4T Engine Oil Degrader. J Bioremed Biodegrad 2:124.doi: 10.4172/2155-6199.1000124 | |
| Copyright: © 2011 Pathak H et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Microorganisms used in all experiments were isolated by selective enrichment technique. MSM broth was used in enrichment technique supplemented with 1% v/v hydrocarbon substrate (4T engine oil). Bacterial inoculation of biometric flask (containing 48 ml minimal salt medium) consisted of 1ml of a 48 hours culture. Hydrocarbon substrate (4T engine oil) was then added to 1% v/v. The GC/MS analysis was performed using a MS 5973 spectrometer coupled to 9 Hewlett Packard model 6890. I4 isolate microorganisms degraded around 76% of 4T engine oil. Gas chromatography showed significant differences in the composition of hydrocarbons at the end of the experiments.
