alexa Alcaligenes-The 4T Engine Oil Degrader | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

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Alcaligenes-The 4T Engine Oil Degrader

Hardik Pathak* and Kamna Bhatnagar
Department of Biotechnology, Mahatma Gandhi Institute of Applied Sciences, Jaipur, India
Corresponding Author : Dr. Hardik Pathak
Head Department of Biotechnology
Mahatma Gandhi Institute of applied sciences, JECRC Campus
Tonk Road, Jaipur, India
Tel: +919602453825
E-mail: [email protected]
Received June 24, 2011; Accepted September 12, 2011; Published September 16, 2011
Citation:Pathak H, Bhatnagar K (2011) Alcaligenes-The 4T Engine Oil Degrader. J Bioremed Biodegrad 2:124.doi: 10.4172/2155-6199.1000124
Copyright: © 2011 Pathak H et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Microorganisms used in all experiments were isolated by selective enrichment technique. MSM broth was used in enrichment technique supplemented with 1% v/v hydrocarbon substrate (4T engine oil). Bacterial inoculation of biometric flask (containing 48 ml minimal salt medium) consisted of 1ml of a 48 hours culture. Hydrocarbon substrate (4T engine oil) was then added to 1% v/v. The GC/MS analysis was performed using a MS 5973 spectrometer coupled to 9 Hewlett Packard model 6890. I4 isolate microorganisms degraded around 76% of 4T engine oil. Gas chromatography showed significant differences in the composition of hydrocarbons at the end of the experiments.

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